剪切应力对生长在微图案表面内皮细胞骨架排列、黏附、迁移和凋亡的影响

发布时间：2018-03-24 16:42

本文选题：内皮细胞　切入点：细胞骨架　出处：《中国组织工程研究》2017年26期

【摘要】：背景:微纳米尺寸的表面修饰法为解决心血管植入物材料表面的内皮化问题提供了一个有效方法。剪切应力在心血管植入物内皮化过程中起着重要作用。目的:综述剪切应力对生长在微图案表面上内皮细胞骨架排列、黏附、迁移和凋亡的影响。方法:作者检索2002至2017年Pub Med数据库和中国知网数据库关于剪切应力对生长在微图案表面上内皮细胞影响的文献,英文检索词为"micropattern,flow shear stress,endothelial cells",中文检索词为"微米拓扑结构,微图案,剪切应力,内皮细胞"。结果与结论:对生长在微图案表面的内皮细胞施加平行剪切应力,可促进微丝沿微图案长轴方向排列,促进内皮细胞沿剪切应力方向迁移,上调内皮细胞FAK的磷酸化水平,增强内皮细胞的黏附能力,并改善内皮细胞的活性。有研究认为平行剪切应力促进微管沿微图案长轴方向排列,另有研究认为平行剪切应力对微管排列无影响。有些文献发现垂直剪切应力破坏生长在微图案表面的内皮细胞的微丝和微管结构,减弱微丝和微管沿微图案长轴方向排列的程度,降低内皮细胞的黏附能力和细胞活性;有些文献发现垂直剪切应力未破坏生长在微图案表面内皮细胞骨架的结构与排列,仍能促进内皮细胞沿剪切应力方向的迁移。此外,剪切力大小影响内皮细胞的迁移,随着剪切应力的增大,生长在微图案表面上的内皮细胞沿剪切应力方向迁移的数量增多。
[Abstract]:Background: surface modification with micro and nanometer size provides an effective method to solve the problem of endothelialization of cardiovascular implants. Shear stress plays an important role in the process of endothelialization of cardiovascular implants. A review of shear stress pair arrangement of endothelial cytoskeleton on micropatterned surfaces, Methods: the effects of Shear stress on Endothelial Cell growth on micropatterned surfaces were retrieved from 2002 to 2017 in Pub Med Database and China Web Database. The English key word is "micropatterning shear stress cells", and the Chinese key word is "micron topology, micropattern, shear stress, endothelial cell". Results & conclusion: parallel shear stress was applied to endothelial cells growing on the surface of micropatterned. It can promote the arrangement of microfilaments along the long axis of micropatterns, promote the migration of endothelial cells along the direction of shear stress, upregulate the phosphorylation level of FAK in endothelial cells, and enhance the adhesion ability of endothelial cells. It is believed that parallel shear stress promotes the arrangement of microtubules along the long axis of micropatterns. Other studies have shown that parallel shear stress has no effect on the arrangement of microtubules. Some studies have found that vertical shear stresses destroy the microfilaments and microtubules of endothelial cells growing on the surface of micropatterns. It was found that vertical shear stress did not destroy the structure and arrangement of endothelial cytoskeleton on the surface of micropatterned endothelial cells. In addition, the magnitude of shear stress affects the migration of endothelial cells. With the increase of shear stress, the number of endothelial cells growing on the micropatterned surface along the direction of shear stress increased.
【作者单位】：北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室;
【基金】：国家自然科学基金(11172031)~~
【分类号】：R318.08

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