柔性全固态无创血糖传感器及3D打印模拟检测装置

发布时间：2018-03-25 20:35

本文选题：柔性葡萄糖传感器　切入点：无创血糖检测　出处：《南京工业大学学报(自然科学版)》2017年06期

【摘要】：基于反向离子电渗透原理,构建无创血糖检测体系。利用丝网印刷工艺制备柔性全固态电极,以普鲁士蓝为电子媒介体,用葡萄糖氧化酶修饰电极制得柔性葡萄糖生物传感器。对传感器的各项修饰条件进行优化,用计时电流法检验其性能,建立起体外葡萄糖溶液定量检测体系。结果表明:在低浓度区对葡萄糖的线性响应范围为0.5~6.5 mmol/L,相关系数(R)为0.993 3,检出限为0.1 mmol/L;高浓度区的线性响应范围为6.5~19.0 mmol/L,相关系数0.992 3。用3D打印制作模拟检测装置,在猪皮上模拟活体电渗透过程进行皮下葡萄糖定量检测。皮下葡萄糖浓度在2.0~14.0 mmol/L范围内,传感器的响应电流与其有较好的相关性(R=0.992 9),浓度范围满足日常血糖检测的需求。为进一步研制实用型无创血糖仪奠定了良好的基础。
[Abstract]:Based on the principle of reverse ion electroosmosis, a noninvasive blood glucose detection system was constructed. Flexible all solid state electrode was prepared by screen printing, and Prussian blue was used as electronic medium. A flexible glucose biosensor was prepared with glucose oxidase modified electrode. The modification conditions of the sensor were optimized and its performance was tested by chronoamperometric method. A quantitative detection system of glucose solution in vitro was established. The results showed that the linear response range of glucose in the low concentration region was 0. 5 渭 mol / L, the correlation coefficient was 0.993 3, the detection limit was 0. 1 mmol / L, and the linear response range of high concentration was 6. 5 ~ 19. 0 mmol / L, phase. The relation number is 0.992 3.The analog detection device is made by 3D printing. The subcutaneous glucose concentration was measured in the range of 2.0 ~ 14.0 mmol/L by simulating in vivo electroosmotic process on pig skin. The response current of the sensor has a good correlation with it, and the concentration range can meet the needs of daily blood glucose detection, which lays a good foundation for the further development of a practical non-invasive blood glucose meter.
【作者单位】：南京工业大学化学与分子工程学院;南京工业大学生物与制药工程学院;南京工业大学化工学院;
【基金】：国家重点基础研究发展计划(973计划)(2013CB733504)
【分类号】：R318.6;TP212.3

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