去细胞输尿管移植至膀胱壁后的再生

发布时间：2018-03-25 23:16

本文选题：组织支架　切入点：再生　出处：《中国临床解剖学杂志》2014年03期

【摘要】：目的通过去细胞技术,将带一部分膀胱壁的大鼠输尿管去细胞后,移植于另一只鼠膀胱壁上,在不重新种植细胞的情况下,利用机体作为"自体反应器"来重构支架,最终使输尿管再生。方法20只SD大鼠的输尿管经过Triton-x100和SDS去细胞后用去离子水漂洗并经过杀菌,支架经过形态学鉴定后移植入其他鼠中,行膀胱修补术同时远端结扎,经过1、2、3、4周获取移植物进行HE染色,免疫荧光法观察分析。结果移植输尿管被分成3个部分:粘附于膀胱的部分(B)、中间部分(M)、游离端(D)。发现4周后B部分有上皮细胞和平滑肌细胞,M部分有脂肪细胞,D部分被纤维化并且在4周后仍然无细胞。B部分在1~2周后,上皮细胞出现,4周后,平滑肌细胞出现。结论未播种输尿管在接合区域也能实现再生,并且先出现上皮层后出现肌层。
[Abstract]:Objective to transplant rat ureteral cells with a part of bladder wall into another rat bladder wall by means of acellular technique, and to reconstruct the scaffold by using the organism as an "autoreactor" without reimplantation of cells. Methods the ureter of 20 Sprague-Dawley rats was treated with deionized water after the removal of cells by Triton-x100 and SDS. The stents were implanted into other rats after morphological identification. Bladder repair was performed and distal ligation was performed. The grafts were harvested for HE staining after 4 weeks of operation. Results the ureter grafts were divided into three parts: the part of the ureter adhered to the bladder, the middle part of the ureter, and the free end of the ureter. It was found that there were epithelial cells in part B and adipocytes in part M of smooth muscle cells 4 weeks later. The D part is fibrotic and is still cell-free 4 weeks later. Part B is 1 to 2 weeks later. Conclusion the unseeded ureter can also regenerate in the junction region and the muscle layer after the appearance of the upper cortex.
【作者单位】：温州医科大学第一临床医学院;温州医科大学基础医学中心;温州医科大学解剖学教研室;
【基金】：浙江省大学生科技创新活动计划“新苗人才计划”(2012R413004) 国家级大学生创新创业训练计划项目(201310343006,201210343008) 浙江省教育厅(NO.Y201327980)
【分类号】：R318.08

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