携重组人骨形态发生蛋白2聚乳酸缓释微球的生物支架复合自体松质骨修复骨缺损

发布时间：2018-03-26 16:59

本文选题：重组人骨形态发生蛋白　切入点：聚乳酸　出处：《现代预防医学》2015年05期

【摘要】：目的探讨含重组人骨形态发生蛋白2(recombinant human Bone Morphogenetic Protein-2,rh BMP-2)聚乳酸(Poly Lactic Acid,PLA)缓释微球的丝素蛋白(Silk Fibroin,SF)/壳聚糖(Chitosan,CS)/纳米羟基磷灰石(nano hydroxyapatite,n-HA)支架复合部分自体松质骨修复骨缺损的疗效。方法取成年新西兰大白兔60只,随机分4组,制备右侧中段桡骨1.5cm的骨缺损模型。A组:含rh BMP-2/PLA缓释微球的SF/CS/n-HA整合支架复合部分自体松质骨,B组:含rh BMP-2的SF/CS/n-HA支架复合部分自体松质骨,C组:单纯自体松质骨,D组:单纯SF/CS/n-HA支架。术后4、8、12周摄X片、并参照Lane-Sandhu骨缺损修复组织X线评分标准,组织学、并参照Lane-Sandhu组织学评分标准进行评分,骨密度检测评估各组骨缺损修复效果。结果术后4、8、12周X片和组织学检查的结果显示,A组与C组比较差异无统计学意义(P0.05),优于其他各组(P0.05)。术后12周骨密度结果示A组与C组比较差异无统计学意义(P0.05),优于其他各组(P0.05)。结论含rh BMP-2/PLA缓释微球的SF/CS/n-HA整合支架复合部分自体松质骨与自体松质骨修复兔桡骨缺损的疗效一致,说明可以大大减少自体松质骨的使用,有望成为一种新型、优良的复合骨组织工程支架材料。
[Abstract]:Objective to investigate the effect of silk fibroin / Chitosanum chitosanum / nano-hydroxyapatiten-HA) scaffold containing recombinant human bone morphogenetic protein 2(recombinant human Bone Morphogenetic protein-2rh BMP-2 (Poly Lactic acid pla) in the repair of bone defects. Methods the bone defects were repaired with self-contained bone scaffolds containing recombinant human bone morphogenetic protein (2(recombinant human Bone Morphogenetic protein-2rh BMP-2). Methods A total of 60 adult New Zealand white rabbits were selected. They were randomly divided into 4 groups, Model of bone defect of right middle radius 1.5cm. Group A: SF/CS/n-HA integrated scaffold with rh BMP-2/PLA sustained-release microspheres combined with partial cancellous bone in group B: SF/CS/n-HA scaffold with rh BMP-2 and partial cancellous bone in C group: simple autogenous cancellous bone D. Group A: SF/CS/n-HA stent alone. X-ray films were taken at 812 weeks after operation. According to the Lane-Sandhu bone defect repair tissue X-ray scoring standard, histology, and referring to the Lane-Sandhu histological score to score, Results the results of X-ray and histological examination showed that there was no significant difference between group A and group C (P 0.05), which was superior to that of other groups (P 0.05). There was no significant difference between group C and group C (P 0.05), which was superior to other groups (P 0.05). Conclusion the effect of SF/CS/n-HA integrated scaffold with Rh BMP-2/PLA sustained release microspheres combined with partial autologous cancellous bone and autologous cancellous bone in repairing rabbit radius defect is the same. The results show that the use of autogenous cancellous bone can be greatly reduced, and it is expected to be a new and excellent composite bone tissue engineering scaffold.
【作者单位】：遵义医学院第三附属医院骨一科;贵州省人民医院骨一科;
【基金】：贵州省省长基金项目(2011(25)),项目名称:《rhBMP-2缓释系统/自体松质骨复合生物支架修复骨缺损的研究》
【分类号】：R687;R318.08

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