不同复温方法对深低温冷冻复合组织血管活性的影响

发布时间：2018-03-31 21:18

  本文选题：深低温保存　切入点：复合组织　出处：《中国医学工程》2012年05期

【摘要】：目的比较3种不同复温方法对深低温冷冻的复合组织中血管活性的影响,找出一种对复合组织中血管损伤最小的复温方法。方法选用20只新西兰大白兔随机分成四组,切取双侧后肢(对照组为新鲜断肢取血管),三个实验组标本经梯度降温后放入液氮中保存7天。分别采用37℃水浴复温组(A组)、空气复温组(B组)、-20℃冰复温组(C组)方法进行复温。采用光镜、免疫组化方法观察血管内皮细胞活性,结果进行统计学分析。结果光镜观察,A组、B组内皮细胞大片脱落,平滑肌细胞变形;而C组内皮细胞和平滑肌细胞保存较好,更接近对照组;免疫组化检测中,3种复温方法血管内皮中均可见VEGF及CD34呈斑片状的弱表达,-20℃冰复温组(C组)VEGF及CD34的表达更接近对照组,高于37℃水浴复温组(A组)及空气复温组(B组)(P0.05)。结论 3种不同复温方法对深低温冷冻的复合组织中血管活性影响不同,-20℃冰复温法影响最小。
[Abstract]:Objective to compare the effects of three different rewarming methods on vascular activity in deep hypothermia and cryopreservation composite tissues, and to find out a rewarming method with minimal vascular damage in the composite tissues. Methods Twenty New Zealand white rabbits were randomly divided into four groups. Bilateral hindlimbs were removed (the control group was fresh amputated limb and blood vessel was removed. The specimens of three experimental groups were kept in liquid nitrogen for 7 days after gradient cooling. They were treated with 37 鈩，

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