便携式定量荧光免疫分析仪

发布时间：2018-04-01 14:12

本文选题：定量　切入点：荧光免疫分析　出处：《仪表技术与传感器》2014年12期

【摘要】：介绍了一种基于荧光免疫与免疫层析技术原理,可对人体血液中C-反应蛋白浓度进行定量检测的便携式荧光免疫分析仪。仪器以MSP430F1611微处理器为主控芯片,配合光电检测模块、机械扫描模块和控制与信息处理模块实现对C-反应蛋白的定量检测。C-反应蛋白与荧光标记物发生免疫反应后聚集在试纸条的检测带上,仪器通过测量检测带上荧光物质的含量,参照标准浓度曲线就可计算出待测样本溶液的浓度。实验数据表明,该仪器C-反应蛋白浓度的线性检测范围为1.00~27.00 mg/L,检测稳定性C.V值7.00%(2.00~20.00 mg/L),最低检测浓度为0.36 mg/L,技术指标达到临床检测的要求。
[Abstract]:Based on the principle of fluorescence immunoassay and immunochromatography, a portable fluorescent immunoanalyzer for quantitative detection of C- reactive protein concentration in human blood is presented in this paper.The instrument takes the MSP430F1611 microprocessor as the main control chip and cooperates with the photoelectric detection module.The mechanical scanning module and the control and information processing module realize the quantitative detection of C- reactive protein. The C- reactive protein and fluorescent marker are collected on the test strip after immune reaction. The instrument measures the content of fluorescent substance in the test strip.The concentration of the sample solution to be measured can be calculated by reference to the standard concentration curve.The experimental data show that the linear detection range of C- reactive protein concentration is 1.00 ~ 27.00 mg / L, the detection stability is 7.00 ~ 2.00 ~ 20.00 mg / L ~ (-1), the lowest detection concentration is 0.36 mg / L, and the technical index is up to the requirement of clinical detection.
【作者单位】：光电信息与传感技术广东普通高校重点实验室(暨南大学);暨南大学光电工程系;
【基金】：国家自然科学基金项目(61177075;11004086;61008057) 广东省战略性新兴产业核心技术攻关项目(2012A032300016) 中央高校基本科研业务费专项资金(21612437;21613405) 广东高校优秀青年创新人才培养计划项目(LYM10024)
【分类号】：R197.39

【参考文献】