量子点与巨噬细胞的生物相容性

发布时间：2018-04-03 23:00

  本文选题：量子点　切入点：巨噬细胞　出处：《中国组织工程研究》2017年26期

【摘要】：背景:与传统的有机荧光染料相比,量子点呈现出良好的生物标记特性,尤其在细胞标记、临床靶向生物成像等领域呈现出独特的光学特性。目的:观察CdS e/Zn S量子点与单核-巨噬细胞的生物相容性。方法:将巨噬细胞RAW264.7接种于96孔板中,分3组培养,分别加入0,50,100 mg/L的Cd Se/Zn S量子点干预,培养1 h或2 h,流式细胞仪检测荧光信号;培养0-24 h,流式细胞仪检测50 mg/L Cd Se/ZnS量子点标记巨噬细胞的荧光信号强度;培养18 h,定量PCR检测巨噬细胞内肿瘤坏死因子α及白细胞介素1β的水平;培养18 h,MTT法检测巨噬细胞增殖,流式细胞仪检测细胞凋亡。结果与结论:(1)荧光信号强度与CdS e/Zn S量子点的质量浓度呈正相关,并且随着标记时间的延长,荧光信号强度增强;(2)经50 mg/L CdS e/ZnS量子点标记后,随着时间的延长,巨噬细胞的荧光信号不断增强,在18 h达到峰值;(3)与0 mg/L量子点组比较,50,100 mg/L量子点组可显著促进巨噬细胞内肿瘤坏死因子α及白细胞介素1β的表达(P0.01或P0.05);100 mg/L量子点组肿瘤坏死因子α表达高于50 mg/L量子点组(P0.01);50,100 mg/L量子点组白细胞介素1β表达无差异;(4)50,100 mg/L Cd Se/Zn S量子点组细胞增殖强于0 mg/L量子点组(P0.05),50,100 mg/L Cd Se/Zn S量子点组细胞增殖无差异;(5)50,100 mg/L Cd Se/Zn S量子点对巨噬细胞的凋亡无明显影响;(6)结果表明,Cd Se/Zn S量子点可活化巨噬细胞,促其增殖与分泌炎症因子,但不影响其凋亡。
[Abstract]:Background: compared with traditional organic fluorescent dyes, quantum dots (QDs) exhibit good biomarker properties, especially in cell labeling, clinical targeted bioimaging and other fields.Aim: to observe the biocompatibility of CdS e/Zn S quantum dots with mononuclear macrophages.Methods: macrophage RAW264.7 was inoculated into 96-well plate and cultured in three groups. The macrophages were incubated with 0 ~ 50100 mg/L CD Se/Zn S quantum dots for 1 h or 2 h, and fluorescence signals were detected by flow cytometry.The fluorescence signal intensity of macrophages labeled with 50 mg/L CD Se/ZnS quantum dots was detected by flow cytometry at 0-24 h, and the levels of tumor necrosis factor 伪 and interleukin-1 尾 in macrophages were measured by quantitative PCR at 18 h.The proliferation of macrophages was detected by MTT assay and apoptosis was detected by flow cytometry.The results showed that the intensity of fluorescence signal was positively correlated with the mass concentration of CdS e/Zn S quantum dots, and the fluorescence signal intensity was enhanced with the prolongation of labeling time.The fluorescence signals of macrophages are increasing.The expression of tumor necrosis factor 伪 and interleukin-1 尾 in macrophages was significantly increased in 50 mg/L QDs group than that in 0 mg/L QDs group. The expression of TNF 伪 in macrophages was significantly higher than that in P0.01 or P0.05 mg/L QDs group.mg/L閲忓瓙鐐圭粍(P0.01);50,100 mg/L閲忓瓙鐐圭粍鐧界粏鑳炰粙绱，

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