国产多孔钽对成骨细胞生物相容性及其相关成骨基因表达的影响

发布时间：2018-04-04 02:42

本文选题：多孔钽　切入点：成骨细胞　出处：《第三军医大学学报》2014年11期

【摘要】：目的探讨国产多孔钽材料对成骨细胞粘附、增殖及成骨基因OPN、OC及FN mRNA表达的影响。方法分离培养新生SD大鼠颅盖骨成骨细胞并鉴定。根据国家标准制备多孔钽浸提液,以多孔钽浸提液与成骨细胞作为实验组,以DMEM完全培养基培养成骨细胞作为对照组。MTT法检测多孔钽浸提液对成骨细胞生长及增殖的影响;倒置相差显微镜及扫描电镜观察成骨细胞在多孔钽上粘附、生长及增殖的情况;实时荧光定量PCR技术检测OPN、OC及FN mRNA的表达并进行定量分析。结果肉眼及扫描电镜观察多孔钽外观呈深灰色、多孔状三维立体结构,表面及断面均匀分布直径400~600μm的微孔,内部孔隙相互连通。MTT法检测结果显示,实验组及对照组成骨细胞培养1~8 d,除第3天外,各时间点细胞光密度值差异无统计学意义(P0.05)。成骨细胞种植于多孔钽培养结果显示,材料表面和孔隙内细胞叠层生长,分泌的骨基质完全覆盖材料表面。实时荧光定量PCR检测结果显示,实验组成骨基因OPN、OC、FN mRNA的相对表达量分别为1.45、1.79、1.69。结论国产多孔钽材料具有良好三维立体空间构型及良好的生物相容性,可刺激成骨细胞的粘附、增殖及成骨基因OPN、OC及FN mRNA的表达。
[Abstract]:Objective to investigate the effects of porous tantalum on osteoblast adhesion, proliferation and expression of osteogenic gene OPNOC and FN mRNA.Methods the osteoblasts of calvaria of newborn SD rats were isolated and identified.The porous tantalum extract was prepared according to the national standard. The effect of porous tantalum extract on the growth and proliferation of osteoblasts was detected by DMEM culture medium as the control group and osteoblast as experimental group.The adhesion, growth and proliferation of osteoblasts on porous tantalum were observed by inverted phase contrast microscope and scanning electron microscope, and the expression of OPNOC and FN mRNA were detected and quantitatively analyzed by real-time fluorescence quantitative PCR.Results the appearance of porous tantalum was dark gray and porous three-dimensional structure was observed by naked eye and scanning electron microscope. The micropores with a diameter of 400 ~ 600 渭 m were evenly distributed on the surface and section. The inner pores were connected with each other by MTT method.In the experimental group and the control group, there was no significant difference in the optical density of osteoblasts between the experimental group and the control group at each time point except the 3rd day (P 0.05).The osteoblasts planted in porous tantalum showed that the surface of the material and the cells in the pores were stacked and the secreted bone matrix completely covered the surface of the material.The results of real-time fluorescence quantitative PCR showed that the relative expression of osteogenic gene OCFN mRNA in the experimental group was 1.45 ~ 1.79 ~ 1.69, respectively.Conclusion porous tantalum has a good three-dimensional spatial configuration and good biocompatibility. It can stimulate osteoblast adhesion, proliferation and the expression of osteogenic gene OPNOC and FN mRNA.
【作者单位】：河北联合大学实验中心;河北联合大学附属医院骨科;河北联合大学基础医学院解剖系;唐山市第二医院关节科;
【基金】：国家科技部科技支撑课题(2012BAE06B03)~~
【分类号】：R318.08

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