蚕蛹羧甲基壳聚糖对小鼠成纤维细胞增殖与生长的影响

发布时间：2018-04-05 18:32

本文选题：蚕蛹羧甲基壳聚糖　切入点：小鼠成纤维细胞L　出处：《蚕业科学》2017年03期

【摘要】：手术后组织粘连与组织损伤部位纤维的增生有密切关系。研究蚕蛹羧甲基壳聚糖对小鼠成纤维细胞L929增殖与生长的影响,探索其用作预防组织粘连生物医用材料的可行性。体外试验结果表明,培养液中添加0.1 mg/m L蚕蛹羧甲基壳聚糖能够极显著抑制小鼠成纤维细胞的增殖(P0.01),抑制率达到50.08%±10.12%;荧光定量PCR检测小鼠成纤维细胞中的细胞生长因子TGF-β1和VEGF编码基因的表达极显著下调(P0.01),并且这种抑制作用呈现明显的剂量效应。据此初步认为,蚕蛹羧甲基壳聚糖可有效控制成纤维细胞中关键的自分泌生长因子编码基因的表达,进而抑制机体的细胞增殖与生长以及炎症反应和血管增生,预防术后组织粘连。
[Abstract]:There is a close relationship between tissue adhesion and fibrous proliferation at the site of tissue injury after operation.To study the effect of silkworm pupae carboxymethyl chitosan on the proliferation and growth of mouse fibroblasts L929 and to explore the feasibility of using it as a biomedical material to prevent tissue adhesion.The results of in vitro experiments showed thatAdding 0.1 mg/m L Carboxymethyl chitosan to the culture medium could significantly inhibit the proliferation of mouse fibroblasts, and the inhibition rate was 50.08% 卤10.12.The fluorescent quantitative PCR was used to detect TGF- 尾 1 and VEGF coding genes in mouse fibroblasts.The expression of P0. 01 was significantly down-regulated, and the inhibitory effect was in a dose-dependent manner.It is concluded that Carboxymethyl chitosan in silkworm pupae can effectively control the expression of key autocrine growth factor-encoding gene in fibroblasts, and then inhibit cell proliferation and growth, inflammatory response and vascular proliferation.Prevention of postoperative tissue adhesion.
【作者单位】：山东省蚕业研究所;
【基金】：山东省农业科学院青年科研基金项目(No.2016YQN61) 山东省现代农业产业技术体系蚕桑创新团队首席兼加工与综合利用岗位专项(No.SDAIT-18-01)
【分类号】：R318.08

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