丝素蛋白的降解性能及其三维打印生物活性支架在皮肤修复中的应用研究

发布时间：2018-04-10 04:14

本文选题：三维打印　切入点：蚕丝蛋白　出处：《浙江大学》2015年硕士论文

【摘要】：丝素蛋白作为一种常用的组织工程支架材料,其降解性能一直不甚清楚,本文从快速降解和酶降解的角度探究和分析了丝素蛋白的降解行为,以及根据降解性能分析结果设计和改良了丝素蛋白材,并用于软组织的修复。发现经过脱胶及后期成型加工处理的丝素蛋白支架的降解速率偏慢,在60天的高温降解以及42天的酶溶液水解后仍有大部分丝素蛋白支架稳定存在。皮肤修复不理想一方面是因为真皮层中缺乏血管的形成,缺少为受伤的皮肤组织提供氧气和营养的渠道,另一方面是由于表皮的再生困难导致伤口难以闭合,需要一个良好的真皮环境促进表皮的迁移及闭合。因此,制备出在生理上能够满足全层皮肤缺损再生要求的活性支架是一项具有挑战性的工作。为了克服这一困难,在这项研究中,我们开发了一个三维打印水凝胶-丝素蛋白支架(3DG-SF),利用修饰在丝素蛋白上的磺酸根(S03)复合碱性成纤维细胞生长因子(FGF-2)形成一个三维的生物活性支架(3DG-SF-SO3-FGF)。利用体外细胞培养和体内全层皮肤缺损的动物模型研究了3DG-SF-SO3-FGF活性支架对大鼠全层皮肤缺损再生的治疗效果。在植入支架14和28天后的全层皮肤缺陷模型的大鼠背部,组织学结果显示与凡士林纱布对照组相比,结合FGF-2的三维打印支架能显著增强皮肤组织的再生。进一步实验表明,结合FGF-2的三维打印支架可能在刺激真皮层中血管形成上具有一定的功能。这可能是FGF-2通过激活真皮血管组织的萌芽提高组织再生。这些发现表明,结合FGF-2的3DG-SF-SO3支架是一种有益皮肤组织工程的支架,在促进皮肤的再生研究上具有一定的应用价值。
[Abstract]:As a commonly used scaffold material for tissue engineering, the degradation performance of silk fibroin is not clear. In this paper, the degradation behavior of silk fibroin was studied and analyzed from the point of view of rapid degradation and enzymatic degradation.The silk fibroin material was designed and improved according to the results of degradation analysis, and was used for soft tissue repair.It was found that the degradation rate of silk fibroin scaffolds treated by degumming and later processing was slow. After 60 days of high temperature degradation and 42 days of hydrolysis of enzyme solution, most of silk fibroin scaffolds remained stable.Skin repair is not ideal because, on the one hand, there is a lack of blood vessel formation in the dermis, a lack of channels to provide oxygen and nutrition to the injured skin tissue, and on the other hand, the wound is difficult to close due to difficulties in the regeneration of the epidermis.A good dermal environment is needed to facilitate the migration and closure of the epidermis.Therefore, it is a challenging task to prepare bioactive scaffolds that can meet the requirements of full-thickness skin defect regeneration.In order to overcome this difficulty, in this study,A three-dimensional printing hydrogel-fibroin scaffold (3DG-SFG) was developed to form a three-dimensional bioactive scaffold (3DG-SF-SO3-FGFG) using sulfonic acid group S03( modified on fibroin protein) combined with basic fibroblast growth factor (FGF-2).The effect of 3DG-SF-SO3-FGF active scaffold on the regeneration of rat full-thickness skin defect was studied by using the animal model of in vitro cell culture and in vivo full-thickness skin defect.The histological results of the full-thickness skin defect model rats after 14 and 28 days of stenting showed that the three-dimensional printing scaffold combined with FGF-2 could significantly enhance the regeneration of skin tissue compared with the control group of vaseline gauze.Further experiments show that the 3D printing scaffold combined with FGF-2 may play a role in stimulating vascular formation in the dermis.It is possible that FGF-2 enhances tissue regeneration by activating the bud of dermal vascular tissue.These findings suggest that the 3DG-SF-SO3 scaffold combined with FGF-2 is a useful scaffold for skin tissue engineering and has certain application value in promoting skin regeneration.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R318.08

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