3D同轴打印组织工程骨支架成型工艺与实验研究

发布时间：2018-04-22 10:19

本文选题：机械式同轴挤出沉积成型 + 组织工程骨支架　；参考：《新疆大学》2017年硕士论文

【摘要】：针对由结核杆菌侵入骨骼或关节引起的骨结核病变,为提高病灶区域的药物利用度,达到高效治疗骨结核病及快速修复骨缺损并进的双重目的,将快速成形技术(RP)、组织工程技术(TE)与生物载药相结合,构建以组织工程骨支架为基础的植入式载药缓释系统。为实现骨结核病药物缓释治疗兼具个性化骨缺损修复提供了全新的思路和有效的方法。本学位论文以同轴复合材料组织工程骨支架的材料选择与同轴结构构建为关键出发点,构建同轴3D打印试验平台与设计同轴喷头挤出装置,研究丝素蛋白(SF)与聚乙烯醇(PVA)的最优配比制备SF/PVA复合水凝胶,并与羟基磷灰石(HA)混合制备SF/PVA/HA复合材料作为同轴支架的内芯增韧材料,同时选用PVA/HA复合材料作为同轴支架的壳层材料。采用机械式同轴挤出快速成形技术制备同轴支架,分析同轴支架打印过程的各工艺参数,研究材料调配比例、同轴喷头比例、扫描速度、挤出速度等因素对同轴支架成形精度的影响,优化关键工艺参数值,制备多种材料复合、宏观形貌良好、孔隙结构均匀且丝材同轴度高的同轴复合材料组织工程骨支架。针对成形支架的成骨潜能、生物相容性进行了细胞毒性实验,证明了本文研究结果的可行性和有效性,并通过成形支架的体外降解实验及动物回植实验验证了支架的降解速率及成骨效能,为以组织工程骨支架为基础的植入式载药缓释系统的研究和应用提供了一定的实验依据。
[Abstract]:In order to improve the drug availability of bone tuberculosis caused by the invasion of tuberculosis bacillus into bone or joint, to achieve the dual purpose of treating bone tuberculosis disease and repairing bone defect quickly. The rapid prototyping (RP) and tissue engineering (TEC) were combined with biological agents to construct an implantable drug delivery system based on tissue engineering bone scaffolds. It provides a new idea and effective method for the treatment of bone tuberculosis with drug release and individualized bone defect repair. Based on the material selection and coaxial structure construction of the coaxial composite tissue engineering bone scaffold, the coaxial 3D printing test platform and the coaxial nozzle extrusion device are constructed in this dissertation. SF/PVA composite hydrogels were prepared by the optimal ratio of silk fibroin (SF) and polyvinyl alcohol (PVA), and SF/PVA/HA composites were mixed with hydroxyapatite (HA) as core toughening materials for coaxial scaffolds. At the same time, PVA/HA composite is used as the shell material of coaxial support. Coaxial bracket was prepared by mechanical coaxial extrusion rapid prototyping technology. The process parameters of coaxial support printing process were analyzed, and the proportion of material blending, coaxial nozzle ratio and scanning speed were studied. The influence of extrusion speed on the forming accuracy of coaxial scaffold was studied. The key process parameters were optimized and many kinds of composite materials were prepared. The microstructure of coaxial composite scaffolds with good macroscopic morphology uniform pore structure and high coaxiality of filaments were obtained. According to the osteogenic potential and biocompatibility of the scaffold, a cytotoxicity test was carried out, which proved the feasibility and effectiveness of the present study. The degradation rate and osteogenic efficiency of the scaffold were verified by in vitro degradation experiments and animal replantation experiments, which provided a certain experimental basis for the research and application of implantable drug delivery sustained release system based on tissue engineering bone scaffold.
【学位授予单位】：新疆大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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