纳米材料（MSNs和MWCNTs）局部应用对小鼠免疫毒性的研究

发布时间：2018-04-23 04:14

本文选题：多壁碳纳米管 + 介孔纳米硅球　；参考：《复旦大学》2012年硕士论文

【摘要】：背景纳米技术在医学领域的应用日益广泛,纳米材料在胰腺癌诊治方面的应用前景也倍受关注,但纳米材料的体内免疫毒性研究甚少。本实验选择两种与胰腺癌治疗关系密切的纳米材料为研究对象(介孔纳米硅球和多壁碳纳米管),探讨其局部给药后对小鼠机体内的免疫毒性反应。方法选用BALB/C雌性小鼠,第一部分检测介孔纳米硅球,第二部分检测多壁碳纳米管。皆随机分为3组,包括高剂量组、低剂量组和对照组,局部爪垫给药后,分别在第14天和第60天检测小鼠免疫功能相关指标的变化。(1)观察一般情况,包括死亡、激惹和嗜睡等；(2)检测小鼠的体重变化、主要脏器系数和淋巴结重量；(3)生化法检测小鼠的血常规和肝肾功能；(4)Elisa法检测小鼠的血清免疫球蛋白、补体C3和炎性细胞因子；(5)组织病理学检查主要脏器的损伤情况；(6)小鼠脾脏T细胞亚群分析；(7)MTT法检测T细胞和B细胞增殖能力。结果(1)实验过程中无小鼠死亡,MSNs和MWCNTs给药部位未见溃疡、糜烂或坏死。(2)MSNs和MWCNTs可能减缓小鼠的体重增长,但不影响小鼠的脏器系数和引流淋巴结重量。(3)MSNs和WMCNTs在观察时间内不引起血常规和肝肾功能的异常的变化。(4)MSNs和MWCNTs在观察时间内未改变IgG、IgM、IgA和补体C3水平,炎性细胞因子在60天时较14天有不同程度下降。(5)所有主要脏器未发现明显的病理性改变。(6)MSNs和MWCNTs不引起小鼠脾脏的T细胞亚群比例异常。(7)MSNs和MWCNTs不影响T细胞和B细胞的增殖能力。结论MSNs与MWCNTs的局部应用可延缓小鼠体重增长,可引起体内的炎性细胞因子水平暂时性的升高,但无明显初级免疫毒性。
[Abstract]:The application of nanotechnology in the field of medicine is becoming more and more extensive, and the prospect of the application of nano materials in the diagnosis and treatment of pancreatic cancer is also concerned. However, there are few studies on the immune toxicity of nanomaterials in vivo. In this experiment, two kinds of nanomaterials, which are closely related to the treatment of pancreatic cancer, were selected as the research on the image (mesoporous nano silicon ball and multi wall carbon nanotube). To discuss the immunological toxicity of local administration to mice.
Methods the BALB/C female mice were used to detect mesoporous nano silicon balls in the first part, and the second parts of the multi wall carbon nanotubes were detected. All of them were randomly divided into 3 groups, including high dose group, low dose group and control group. After the local claw pad was administered, the changes of immune function related indexes were detected in Fourteenth days and sixtieth days respectively. (1) the general situation, including death, was observed. (2) test the body weight change, main organ coefficient and lymph node weight (3) biochemical test of blood routine and liver and kidney function of mice; (4) Elisa method to detect the serum immunoglobulin, complement C3 and inflammatory cytokines in mice; (5) pathological examination of major organ damage; (6) T fine spleen of mice Analysis of cell subsets; (7) MTT assay was used to detect the proliferation ability of T cells and B cells.
Results (1) no mice died during the experiment. There was no ulcer, erosion or necrosis of MSNs and MWCNTs. (2) MSNs and MWCNTs could slow the weight growth of mice, but did not affect the organ coefficient and the weight of drainage lymph nodes. (3) MSNs and WMCNTs did not cause abnormal changes of blood routine and liver and kidney function during the observation time. (4) MSNs and M WCNTs did not change the levels of IgG, IgM, IgA and complement C3 during the observation time. The inflammatory cytokines were decreased in different degrees at 60 days. (5) no obvious pathological changes were found in all the main organs. (6) MSNs and MWCNTs did not cause the abnormal proportion of T cell subsets in the spleen of mice. (7) MSNs and MWCNTs did not affect the proliferation of T and B cells.
Conclusion the local application of MSNs and MWCNTs can delay the weight growth of mice, and can cause a temporary increase in the level of inflammatory cytokines in the body, but there is no obvious primary immuno toxicity.

【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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