损伤角膜修复重建中的ROCK抑制剂:促进角膜细胞增殖、迁移和黏附

发布时间：2018-05-02 00:55

本文选题：角膜 + 内皮细胞　；参考：《中国组织工程研究》2015年33期

【摘要】：背景:随着人们对Rho/ROCK通路的生物学功能的了解的不断加深,新的ROCK抑制剂不断被发现,且ROCK抑制剂在角膜细胞存活、增殖和迁移中表现出良好的促进效果,ROCK抑制剂的研究能为再生医学和临床细胞移植提供更多的良好供体材料或种子细胞。目的:总结并探讨ROCK抑制剂Y-39983和Y-27632在角膜病的治疗和应用的研究进展。方法:应用计算机检索Pub Med数据库和中国知网数据库内相关文章,检索时间为2008至2015年,检索词为"corneal endothelial cell,corneal epithelial cell,ROCK inhibitor,Y-39983,Y-27632,ROCK抑制剂,角膜",从中筛选出与主题相关的部分文献。结果与结论:初检得到264篇文章,按主题相关性对文献进一步筛选,最终纳入45篇文章进行讨论。应用到眼科的ROCK抑制剂主要有两种:Y-27632和Y-39983,两者的应用主要还是基础研究与临床试验阶段。Y-27632可促进角膜缘上皮干细胞增殖及提高冻存复苏后的活性;Y-39983作为一种新型的ROCK抑制剂,其比Y-27632更有效地抑制ROCK激酶的活性,从而能更有效地促进角膜内皮的愈合。目前ROCK抑制剂在角膜治疗的研究较多,但是没有建立一种稳定的获得种子细胞的方法,每一种治疗方法都各有优缺点,而克服这些缺点以及找到快速稳定获得种子细胞的方法是今后的发展方向。
[Abstract]:Background: with the increasing understanding of the biological functions of the Rho/ROCK pathway, new ROCK inhibitors have been discovered and ROCK inhibitors have survived in corneal cells. The study of rock inhibitors in proliferation and migration can provide more good donor materials or seed cells for regenerative medicine and clinical cell transplantation. Objective: to summarize and discuss the progress in the treatment and application of ROCK inhibitors Y-39983 and Y-27632 in corneal diseases. Methods: the relevant articles in Pub Med database and Chinese knowledge network database were searched by computer. The search time was from 2008 to 2015. The key words were "corneal endothelial cell corneal epithelial cell roll inhibitor Y-39983Y -27632rock inhibitor, cornea". Some articles related to the subject were selected from the search time. Results & conclusion: 264 articles were collected and further selected according to the thematic correlation. Finally, 45 articles were included for discussion. There are mainly two kinds of ROCK inhibitors used in ophthalmology: 1: Y-27632 and Y-39983.The main applications of these inhibitors are basic research and clinical trials. Y-27632 can promote the proliferation of limbal epithelial stem cells and increase the activity of cryopreservation and resuscitation as a new type of ROCK inhibitor. It can inhibit the activity of ROCK kinase more effectively than Y-27632, thus it can promote corneal endothelium healing more effectively. At present, there are many studies on ROCK inhibitors in corneal therapy, but there is no stable method to obtain seed cells, and each treatment has its own advantages and disadvantages. Overcoming these shortcomings and finding ways to obtain seed cells quickly and steadily are the future development direction.
【作者单位】：暨南大学再生医学教育部重点实验室;暨南大学生命科学技术学院生物技术系;暨南大学医学院眼科研究所;
【基金】：国家自然科学基金赞助(81371689)~~
【分类号】：R318.08

【参考文献】