载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用

发布时间：2018-05-02 02:09

本文选题：羟基磷灰石 + 胶原　；参考：《中国临床解剖学杂志》2014年05期

【摘要】：目的研制载阿霉素(ADM)的聚乳酸-羟基乙酸共聚物(PLGA)纳米微球的纳米羟基磷灰石/胶原复合支架(ADM-PLGA-NHAC),研究其性质及体外释药特点,探讨其体外抑制人骨肉瘤MG63细胞的作用,为骨肉瘤的治疗提供新策略。方法以纳米羟基磷灰石及胶原为原料制备纳米羟基磷灰石/胶原支架并在其中加载ADM-PLGA纳米微球,通过扫描电子显微镜、体外释放行为等手段评价载药支架材料的性能。以CCK8法、活-死染色评价该复合支架的浸提液在体外对人骨肉瘤MG63细胞株的抗肿瘤活性。结果复合支架的孔径多在100~200μm,孔隙率约为82%,微球与支架间结合较为紧密。复合支架具有良好的缓释特性,28 d内能持续缓慢释放阿霉素。复合支架的浸提液对骨肉瘤MG63细胞生长有明显的抑制作用。结论制备的ADM-PLGA-NHAC复合支架具有良好的药物缓释特性及抗肿瘤效应,是一种具有良好应用前景的抗骨肉瘤骨修复材料。
[Abstract]:Objective to develop a nano hydroxyapatite / collagen composite scaffold (ADM-PLGA-NHAC) containing ADM (PLGA) nanospheres, study its properties and release characteristics in vitro, explore the effect of its in vitro inhibition of human osteosarcoma MG63 cells, and provide a new strategy for the treatment of osteosarcoma. The nano hydroxyapatite / collagen scaffold was prepared with collagen and ADM-PLGA nanospheres were loaded in it. The properties of the drug carrier were evaluated by scanning electron microscope and in vitro release behavior. The antitumor activity of the extract of the composite scaffold on human osteosarcoma MG63 cell line was evaluated by CCK8 method. Results the pore size of the composite scaffold is 100~200 mu m, the porosity is about 82%, and the combination of the microspheres and the scaffold is closer. The composite scaffold has good sustained release characteristics and the 28 d internal energy is slowly releasing adriamycin. The extracts of the composite scaffold have obvious inhibitory effect on the growth of osteosarcoma MG63 cells. Conclusion ADM-PLGA-NHAC composite scaffold has been prepared. It has good drug release characteristics and anti-tumor effect. It is a promising osteosarcoma bone repair material with good application prospects.

【作者单位】：南方医科大学珠江医院骨科中心;
【基金】：广州市科技民生科技重大专项(2011Y2-00023-2)
【分类号】：R318.08

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