羧化壳聚糖止血及其生物安全性评价

发布时间：2018-05-03 21:42

本文选题：壳聚糖 + 止血　；参考：《中国组织工程研究》2017年22期

【摘要】：背景:羧化壳聚糖是一类具有优良应用性能的壳聚糖衍生物,良好的生物性能使其在止血材料中具有安全有效的优势。目的:探讨羧化壳聚糖的止血效果及其生物安全性。方法:(1)实验组分别在SD大鼠尾静脉出血创面及肝脏出血创面覆盖羧化壳聚糖粉末,记录止血时间,以创面自然止血时间为对照;(2)实验组分别在ICR小鼠断尾创面、股动脉出血创面及肝脏出血创面覆盖羧化壳聚糖粉末,记录止血时间,以创面自然止血时间为对照;(3)将羧化壳聚糖糊状物、十二烷基硫酸钠溶液及蒸馏水分别直接敷于白化兔皮肤上,进行皮肤刺激实验;(4)在豚鼠皮内注射羧化壳聚糖溶液,进行迟发型超敏反应实验;(5)在白化兔皮下分别注射羧化壳聚糖生理盐水溶液、生理盐水,进行皮内刺激实验;在白化兔皮下分别注射羧化壳聚糖浸提于食用橄榄油的上清液及食用橄榄油,进行皮内刺激实验。结果与结论:(1)与对照组比较,实验组SD大鼠尾静脉、肝脏出血创面止血时间明显缩短(P0.01);(2)与对照组比较,实验组ICR小鼠断尾创面、股动脉出血创面及肝脏出血创面止血时间明显缩短(P0.05或P0.01);(3)羧化壳聚糖对白化兔皮肤无刺激性,具有极轻微的白化兔皮内刺激反应;羧化壳聚糖对豚鼠皮肤无致敏反应;(4)结果表明,羧化壳聚糖对SD大鼠、ICR小鼠创面具有较好的止血作用,对白化兔及豚鼠无明显皮肤刺激、致敏反应及皮内刺激反应,是一种较安全的止血材料。
[Abstract]:Background: carboxylated chitosan is a kind of chitosan derivatives with excellent application performance. Its good biological properties make it safe and effective in hemostatic materials. Objective: to study the hemostatic effect and biological safety of carboxylated chitosan. Methods 1) the experimental group covered with carboxylated chitosan powder on the wound of caudal vein hemorrhage and hepatic hemorrhage in SD rats, recorded the hemostatic time, and took the natural hemostatic time of the wound as the control. The carboxylated chitosan powder was covered in the wound of femoral artery hemorrhage and hepatic hemorrhage, and the hemostatic time was recorded, and the carboxylated chitosan paste was used as the control. Sodium dodecyl sulfate solution and distilled water were applied directly to the albino rabbit skin respectively. The delayed-type hypersensitivity test was carried out. The intradermal stimulation was carried out by injecting carboxylated chitosan saline solution and normal saline under the albino rabbit skin. The supernatant and olive oil extracted from olive oil by carboxylation chitosan were injected under albino rabbit skin, and the experiment of intradermal stimulation was carried out. Results compared with the control group, the hemostasis time of the experimental group was significantly shorter than that of the control group (P 0.01). Compared with the control group, the experimental group ICR mice had severed tail wound. The hemostasis time of femoral artery bleeding wound and liver hemorrhage wound was significantly shortened by P0.01P0.01H0. 05) carboxylation chitosan had no irritation to albino rabbit skin and had very slight intradermal stimulation response. The results showed that carboxylated chitosan had a good hemostatic effect on the wound of SD rat ICR mice, and had no obvious skin irritation, sensitizing reaction and intradermal stimulation on white rabbits and guinea pigs. Is a relatively safe hemostatic material.
【作者单位】：浙江省中医药研究院;
【基金】：浙江省科技厅项目(2015F50067)~~
【分类号】：R318.08

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