兔膝人工关节假体纳米银复合涂层抗菌性能的实验研究

发布时间：2018-05-07 01:38

本文选题：羟基磷灰石 + 纳米银　；参考：《新疆医科大学》2015年硕士论文

【摘要】：目的：探讨制备人工关节假体表面纳米银复合涂层材料的最佳参数,观测银离子在模拟体液中的缓释性能,评价复合材料组织相容性及动物体内的抗菌性能。方法：应用脉冲电化学方法,在钛金属表面沉积羟基磷灰石/纳米银复合涂层,并对其形貌、空间构象、组成进行表征分析。将材料浸泡在模拟体液中,采用原子能吸收光谱法,检测不同时间点溶液银离子浓度。按照按国家和ISO的检测标准,进行组织相容性实验。成年新西兰兔48只,随机分为实验A、B、C三组(n=16)。行兔全膝关节置换术,实验A、B组植入不同银离子浓度电解液沉积的人工关节假体复合材料,实验C组植入普通人工关节假体。建立兔全膝关节置换术后金黄色葡萄球菌感染模型,评价复合材料在动物体内抗菌性能。结果：0.5HA/Ag复合涂层银含量为2.75wt%；第1d银离子释放量占30d总量的45.55%；溶血率为1.64%,体外对金黄色葡萄球菌的抗菌率为95.3%。1.0HA/Ag复合涂层银含量为8.83wt%；第1d银离子释放量占30d总量的48.10%；溶血率为2.24%；体外对金黄色葡萄球菌的抗菌率为97.6%。动物感染模型中,实验A、B组体温、血C-反应蛋白、血沉、血浆降钙素原均低于实验C组,组间差异有统计学意义(P0.05)。感染率：实验A组18.75%(3/16),实验B组12.50%(2/16),实验C组100%(16/16),组间差异有统计学意义(P0.05)；实验A、B组之间感染率差异无统计学意义(P0.05),实验组与对照组比较,感染率差异有统计学意义(P0.05)。结论：羟基磷灰石/纳米银复合涂层是由纳米针状羟基磷灰石和点状的银颗粒组成,银颗粒分布均匀,无团聚现象。在观察期间,复合材料的缓释作用可维持有效的抗菌浓度,释放总量低于细胞的毒性浓度。纳米银复合钛涂层材料组织相容性良好。体外对金黄色葡萄球菌的抗菌率在95%以上。羟基磷灰石/纳米银复合涂层人工关节假体在动物体内对金黄色葡萄球菌具有良好的抗菌活性。
[Abstract]:Aim: to investigate the optimal parameters for preparing nano-silver composite coating materials on artificial joint prosthesis, observe the slow release properties of silver ions in simulated body fluid, and evaluate the tissue compatibility and antibacterial properties of the composite materials in vivo. Methods: the hydroxyapatite / nano-silver composite coating was deposited on the surface of titanium by pulsed electrochemical method. The morphology, spatial conformation and composition of the coating were characterized. The material was immersed in simulated body fluid and the concentration of silver ions in solution at different time points was determined by atomic absorption spectrometry. According to the national and ISO standards, the histocompatibility test was carried out. A total of 48 adult New Zealand rabbits were randomly divided into three groups: group A (n = 16). Total knee arthroplasty was performed in rabbits. In group A, artificial joint composites deposited with different silver ion concentration electrolyte were implanted, and in group C, common artificial joint prostheses were implanted. A rabbit model of staphylococcus aureus infection after total knee arthroplasty was established to evaluate the antibacterial properties of composite materials in vivo. Results the silver content of 0.5 HA / Ag composite coating was 2.75 wtt.The silver ion release in the first day accounted for 45.55% of the total amount in 30 days, the hemolysis rate was 1.64 and the antibacterial rate against Staphylococcus aureus in vitro was 8.83 wt.The silver release amount in the first day accounted for 48.10% of the total amount of silver ion release in 30 days. The hemolysis rate was 2.24% and the antibacterial rate against Staphylococcus aureus in vitro was 97.6%. In the model of animal infection, the body temperature, serum C-reactive protein, erythrocyte sedimentation rate and plasma procalcitonin in group A were lower than those in group C, and the difference between the two groups was statistically significant (P 0.05). The infection rate of group A was 18.75 / 16, that of group B was 12.50 / 16, that of group C was 100 / 16 / 16, and that of group A was not significantly different from that of group A (P 0.05), and that of group A was significantly higher than that of group B (P 0.05). Conclusion: hydroxyapatite / nano-silver composite coating is composed of needle-like hydroxyapatite and dot silver particles with uniform distribution and no agglomeration. During the observation period, the sustained release of the composite could maintain the effective antibacterial concentration and the total release amount was lower than the cytotoxic concentration. The nano-silver composite titanium coating material has good histocompatibility. The antibacterial rate against Staphylococcus aureus in vitro was over 95%. Hydroxyapatite / nano-silver composite coating artificial joint prosthesis has good antibacterial activity against Staphylococcus aureus in vivo.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R318.17

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