壳聚糖即型水凝胶的理化性质、止血功能和生物相容性研究

发布时间：2018-05-10 09:33

本文选题：壳聚糖 + 水凝胶　；参考：《功能材料》2014年09期

【摘要】：报道了壳聚糖即型水凝胶,通过红外光谱和扫描电镜对该水凝胶进行了结构分析与表征,测定了其基本理化性质,并探讨了其止血功能和生物安全性。该水凝胶的红外光谱证实了西弗碱反应的发生,证明该水凝胶是由羟丙基壳聚糖和改性海藻酸钠交联而成;SEM照片显示该水凝胶的骨架是连续均一的蜂窝状网络结构;该水凝胶成胶迅速,最短成胶时间为10s,凝胶强度约为50g/cm2,溶胀率约为14.7%,pH值为7.5。在大鼠肝脏损伤出血模型的止血实验中,壳聚糖水凝胶具有优异的止血功能,止血时间和出血量均优于空白对照组(P0.01),与纤维蛋白胶组无显著性差异(P0.05)。壳聚糖水凝胶对细胞生长无明显抑制作用,其在体内降解伴随着组织炎症反应,炎症反应程度随材料的降解而减轻。
[Abstract]:Chitosan instant hydrogel was reported. The structure of the hydrogel was analyzed and characterized by infrared spectroscopy and scanning electron microscope. The physical and chemical properties of the hydrogel were determined. The hemostatic function and biological safety of the hydrogel were also discussed. The infrared spectra of the hydrogel confirmed the occurrence of sieverine reaction. It was proved that the hydrogel was crosslinked by hydroxypropyl chitosan and modified sodium alginate. The SEM photos showed that the framework of the hydrogel was a continuous homogenous honeycomb network structure. The hydrogel formed quickly, the shortest gelation time was 10s, the gel strength was about 50g / cm ~ 2, the swelling rate was about 14.7g / cm ~ (-2) and the pH value was 7.5. In the hemostatic experiment of rat liver injury and hemorrhage model, chitosan hydrogel had excellent hemostatic function, the hemostasis time and the amount of bleeding were better than that of the blank control group (P0.01), but there was no significant difference between the chitosan hydrogel group and the fibrin gel group (P0.05). Chitosan hydrogel had no obvious inhibitory effect on cell growth. The degradation of chitosan hydrogel was accompanied by tissue inflammation in vivo, and the degree of inflammation decreased with the degradation of the material.
【作者单位】：中国海洋大学海洋生命学院;青岛博益特生物材料有限公司;
【基金】：国家高技术研究发展计划(863计划)资助项目(2014AA093605)
【分类号】：R318.08;O648.17

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