两种壳聚糖基复合水凝胶的比较研究

发布时间：2018-05-18 12:10

本文选题：磷酸钙陶瓷材料 + 壳聚糖　；参考：《西南交通大学》2012年硕士论文

【摘要】：骨缺损是常见的临床疾病,多是由于创伤、感染、肿瘤等所导致,通常需要进行骨移植治疗。目前主要的治疗方案有自体骨移植、异体骨移植、骨组织工程支架。其中骨组织工程支架最为引人注目。磷酸钙类陶瓷材料是最常见的骨组织工程支架材料之一,由于高致密度的磷酸钙类陶瓷材料在体内降解很慢,人们常常通过溶胶凝胶等方法制备高孔隙率和高贯通性的可降解磷酸钙类陶瓷材料,不少复合高分子凝胶是良好的缓释材料,具有较好的生物相容性,可以复合到外层孔壁上,进一步改善提高磷酸钙陶瓷材料的生物学性质。而复合凝胶材料的设计和选择是关键。壳聚糖是自然界中广泛存在的天然多糖,具有良好的生物相容性和可降解性。壳聚糖基水凝胶是目前研究最多的水凝胶材料之一。本课题以琥珀酸酐改性改善了壳聚糖的水溶性,通过高碘酸钠氧化了结冷胶和海藻酸钠,用可溶性壳聚糖和生成的氧化产物反应分别制备了两种壳聚糖基水凝胶。通过考察对比其凝胶时间、溶胀性能、降解性能等性能并与成纤维细胞和成骨细胞共培养后发现,两种复合水凝胶都具有良好的生物相容性和降解性,同时N-琥珀酰壳聚糖/氧化结冷胶复合水凝胶的溶胀性能,降解性能,生物相容性都要优于N-琥珀酰壳聚糖/氧化海藻酸钠复合水凝胶。更加适合作为凝胶材料复合至磷酸钙陶瓷材料上
[Abstract]:Bone defect is a common clinical disease, usually caused by trauma, infection, tumor and so on. At present, the main treatment options are autologous bone transplantation, allograft bone graft, bone tissue engineering scaffold. Bone tissue engineering scaffolds are most attractive. Calcium phosphate ceramic material is one of the most common scaffold materials for bone tissue engineering, because the high density calcium phosphate ceramic material degrades very slowly in vivo. Biodegradable calcium phosphate ceramics with high porosity and high permeability are often prepared by sol-gel methods. Many composite polymer gels are good sustained-release materials with good biocompatibility and can be compounded to the outer pore wall. Further improve the biological properties of calcium phosphate ceramic materials. The design and selection of composite gel materials is the key. Chitosan is a kind of natural polysaccharide, which has good biocompatibility and biodegradability. Chitosan-based hydrogel is one of the most studied hydrogel materials. In this paper, the water solubility of chitosan was improved by the modification of succinic anhydride. Two chitosan based hydrogels were prepared by sodium periodate oxidation of cold glue and sodium alginate. The gel time, swelling and degradation properties were compared and co-cultured with fibroblasts and osteoblasts. The results showed that the two hydrogels had good biocompatibility and biodegradability. At the same time, the swelling, degradation and biocompatibility of N- succinyl chitosan / oxidized cold gel were better than those of N- succinyl chitosan / sodium alginate composite hydrogels. It is more suitable for composite as gel material to calcium phosphate ceramic material.
【学位授予单位】：西南交通大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

【参考文献】