多孔钽金属与成骨细胞生物相容性的实验研究

发布时间：2018-05-18 21:48

本文选题：多孔钽金属 + 成骨细胞　；参考：《中国矫形外科杂志》2014年12期

【摘要】：[目的]研究多孔钽金属体外与成骨细胞的生物相容性。[方法]按照ISO10993-12医疗器械生物学评价标准制备多孔钽金属材料浸提液,作用于人成骨细胞系hFOB1.19,标记为实验组,并设置空白对照组;MTT法检测材料浸提液对成骨细胞生长的影响;ELISA酶联免疫吸附法检测实验组及对照组碱性磷酸酶ALP及骨钙素OCN活性;将成骨细胞与多孔钽金属共培养,扫描电镜观察成骨细胞在材料上黏附和生长情况。[结果]实验组和对照组细胞增殖曲线、ALP和OCN活性相似,差异无统计学意义(P0.05);成骨细胞与多孔钽金属材料共培养,显现出良好的黏附和生长行为。[结论]多孔钽金属对成骨细胞生长无不良影响,且适于成骨细胞黏附、生长及分化,具有良好的生物相容性。
[Abstract]:[Objective] to study the biocompatibility of porous tantalum in vitro and osteoblast. [Methods] the preparation of porous tantalum material leaching solution was prepared according to the biological evaluation standard of ISO10993-12 medical instrument, which was used as an experimental group and set up a blank control group, which was used as the human osteoblast line hFOB1.19, and the MTT method was used to detect the growth of osteoblast by the material leaching solution. ELISA enzyme linked immunosorbent assay was used to detect the activity of alkaline phosphatase ALP and osteocalcin OCN in the experimental group and the control group. The osteoblasts were co cultured with the porous tantalum metal, and the adhesion and growth of the osteoblasts were observed by scanning electron microscope. [results] the cell proliferation curve of the experimental group and the control group was similar to the activity of ALP and OCN, and the difference was not statistically significant. P0.05); osteoblasts and porous tantalum materials co cultured to show good adhesion and growth behavior. [Conclusion] porous tantalum has no adverse effects on the growth of osteoblasts, and is suitable for osteoblast adhesion, growth and differentiation, and has good biocompatibility.
【作者单位】：厦门大学附属福州第二医院骨科;
【基金】：福建省自然科学基金项目(编号:2009J05164)
【分类号】：R318.08

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