内皮细胞细胞外基质改性钛表面内皮细胞和血小板的生长行为

发布时间：2018-05-20 20:45

本文选题：碱活化 + 细胞外基质　；参考：《科学技术与工程》2014年25期

【摘要】：实验结合碱活化和钛表面原位构建人脐静脉内皮细胞(HUVECs)细胞外基质(extracellular matrix,ECM)的方法制备细胞外基质改性的粗糙钛片(Ti-OH-ECM),然后比较粗糙钛(Ti-OH)表面和光滑钛(Ti,对照组)表面构建ECM前后的表面形貌、亲疏水性变化、官能团表征、细胞相容性和血液相容性。结果表明,ECM在钛表面构建成功,粗糙钛表面接触角降低至9°,ECM改性后,粗糙钛片的接触角有显著的增高,具有统计学意义(P0.01);并且ECM在粗糙钛表面沉积的量显著大于对照组表面(P0.01);1 d和3 d的细胞荧光染色结果表明,粗糙钛表面抑制内皮细胞黏附,但是ECM改性后的粗糙钛表面内皮细胞黏附数量高于对照组改性后的表面,并且具有统计学意义(P0.05);与粗糙钛表面比较,ECM改性后的粗糙钛促进平滑肌细胞粘附及增殖;另外,2 h的血小板黏附结果表明,与对照组相比,粗糙钛的血小板黏附数量显著减少(P0.01),ECM改性后的粗糙钛表面进一步抑制血小板黏附及激活(P0.05)。因此,细胞外基质改性的碱活化钛为生物医疗器械提供了一定的研究基础。
[Abstract]:In combination with alkaline activation and in situ construction of human umbilical vein endothelial cells (HUVECs) extracellular matrix ECM (ECM), the rough Ti-OH-ECM modified by extracellular matrix was prepared, and the rough Ti-OH) surface was compared with that of smooth titanium (control group). The surface morphology before and after ECM was constructed. Changes in hydrophobicity, characterization of functional groups, cytocompatibility and blood compatibility. The results showed that the ECM was successfully constructed on the titanium surface, and the contact angle of the rough titanium surface increased significantly when the contact angle of the rough titanium surface was reduced to 9 掳ECM. The amount of ECM deposited on the rough surface of titanium was significantly higher than that on the surface of the control group for 1 d and 3 d. The results showed that the surface of rough titanium inhibited the adhesion of endothelial cells. However, the number of endothelial cell adhesion on the surface of rough titanium modified by ECM was higher than that on the surface of the control group, and there was a statistically significant difference in the adhesion and proliferation of smooth muscle cells between the modified surface of ECM and the surface of rough titanium, and the surface of rough titanium modified by ECM could promote the adhesion and proliferation of smooth muscle cells. In addition, the results of platelet adhesion for 2 h showed that, compared with the control group, the amount of platelet adhesion of rough titanium decreased significantly, and the surface of rough titanium modified by ECM further inhibited platelet adhesion and activated P0.05. Therefore, alkali-activated titanium modified by extracellular matrix provides a basis for biomedical devices.
【作者单位】：西南交通大学材料科学与工程学院;
【分类号】：R318.08

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