高仿真壳聚糖支架修复腹壁缺损

发布时间：2018-05-21 09:10

本文选题：壳聚糖 + 聚丙烯类　；参考：《中国组织工程研究》2017年26期

【摘要】：背景:研究显示,壳聚糖能提高纤维细胞增殖、迁移速度,促进细胞外基质的合成,能为缺损部位营造良好的愈合环境。目的:观察高仿真壳聚糖支架修复大鼠腹壁缺损的效果。方法:取成年SD大鼠60只,建立腹壁缺损模型,随机分2组修复,实验组于缺损部位植入高仿真壳聚糖支架,对照组植入聚丙烯补片,修复后2,4周,进行修复部位大体、粘连及病理组织学观察。结果与结论:(1)大体观察:对照组修复后2周缺损部位与网膜存在粘连现象,修复4周后补片部位明显增厚,与周围组织界限比较清晰,补片区域色泽一般,整合一般;实验组修复2周后缺损部位与支架轻微粘连,修复后4周补片增厚,缺损部位未见明显隆起、红肿、感染,伤口愈合较好;(2)粘连:实验组修复后4周Kadata粘连评分低于对照组(P0.05);(3)病理组织学观察:对照组修复4周后可见毛细血管生长,存在大量的成纤维细胞,缺损部位存在大量炎细胞浸润;实验组修复4周后可见少许炎症细胞,存在大量胶原纤维、毛细血管,肉芽组织成熟度较高;(4)结果表明:高仿真壳聚糖支架可促进缺损腹壁的修复,减轻炎性反应。
[Abstract]:Background: chitosan can improve the proliferation and migration of fibroblasts, promote the synthesis of extracellular matrix, and create a good healing environment for the defect site. Objective: to observe the effect of high-simulated chitosan scaffold in repairing abdominal wall defect in rats. Methods: 60 adult SD rats were selected to establish abdominal wall defect model, and were randomly divided into 2 groups. The experimental group implanted high simulated chitosan scaffold in the defect site, the control group implanted polypropylene patch, 2 weeks after repair, the repair site was gross. Adhesion and histopathological observation. Results and conclusion: in the control group, there was adhesion between the defect and omentum 2 weeks after repair. After 4 weeks of repair, the patch was thickened, and the boundary between the patch and the surrounding tissue was clear, the color of the patch was general and the integration was general. In the experimental group, there was slight adhesion between the defect site and the stent 2 weeks after repair, and the thickness of the patch increased 4 weeks after repair. No significant swelling, swelling and infection were found in the defect site. The Kadata adhesion score in the experimental group was lower than that in the control group at 4 weeks after repair (P 0.05). Histopathological observation: capillary growth was observed in the control group after 4 weeks of repair, and a large number of fibroblasts were found in the experimental group. There were a large number of inflammatory cells infiltrating in the defect site, a few inflammatory cells were found in the experimental group after 4 weeks of repair, and a large number of collagen fibers and capillaries were found in the experimental group. The results showed that high imitated chitosan scaffold could promote the repair of the abdominal wall and alleviate the inflammatory reaction.
【作者单位】：青海省肿瘤医院;河北医科大学第三医院;
【基金】：河北省科技厅项目(20110426)~~
【分类号】：R318.08;R656

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