新型多孔钽金属支架材料的生物学评价

发布时间：2018-05-25 23:15

本文选题：材料试验 + 钽　；参考：《中国组织工程研究》2017年18期

【摘要】：背景:利用涂层制备技术在传统材料基体上沉积金属钽涂层,既利用了金属钽优异生物学性能又可降低成本,为金属钽器件的应用提供了一条切实可行的方向。目的:制备金属钽涂层支架材料,观察其体内外生物相容性。方法:采用常压化学气相沉积技术在多孔碳化硅基体上制备多孔金属钽涂层支架材料,进行以下实验:(1)体外实验:将骨髓间充质干细胞与多孔金属钽涂层支架材料共培养2周,MTT法检测细胞增殖;培养第5,10,15天,扫描电镜观察细胞在材料表面附着情况;(2)体内实验:在犬股骨头骨缺损处植入多孔钽材料,分别于植入后6,12周处死实验动物,将标本硬组织切片染色后,显微镜下观察多孔钽材料与周围组织的生长情况。结果与结论:(1)体外实验结果:随着培养时间的延长,骨髓间充质干细胞呈现出极其旺盛的增殖,相互排列紧密,细胞之间连接并爬行生长进入多孔钽孔隙中,细胞完全覆盖在多孔钽表面,细胞与细胞之间形成团状重叠现象;(2)体内实验结果:植入6周,材料与骨组织周围界限清晰,多孔钽孔隙中有少量骨组织爬行,有少许骨小梁长入,未附着部分可见孔隙周围空虚及缝隙,材料周围未见组织破坏及排斥反应;植入12周,可明显观察到材料与骨组织生长良好,多孔钽表面和孔隙内大量骨组织长入,材料孔隙与组织紧密连接,有大量骨小梁长入,多孔钽与骨组织融于一体;(3)结果表明:采用常压化学气相沉积技术制备的金属钽涂层支架材料具有良好的生物相容性。
[Abstract]:Background: metal tantalum coating is deposited on traditional materials by coating preparation technology, which not only makes use of the excellent biological properties of tantalum but also reduces the cost, which provides a practical direction for the application of metal tantalum devices. Objective: to study the biocompatibility of tantalum coated scaffolds in vivo and in vitro. Methods: porous metal tantalum coated scaffolds were prepared on porous silicon carbide substrate by atmospheric pressure chemical vapor deposition. In vitro, bone marrow mesenchymal stem cells and porous tantalum coated scaffolds were co-cultured for 2 weeks to detect cell proliferation. Scanning Electron Microscopy (SEM): porous tantalum was implanted into the defect of canine femoral head. The animals were killed at 612 weeks after implantation, and the specimens were stained with hard tissue sections. The growth of porous tantalum and its surrounding tissues were observed under microscope. Results and conclusion: with the extension of culture time, bone marrow mesenchymal stem cells (BMSCs) showed extremely vigorous proliferation and close arrangement, and the cells were connected and crawled into porous tantalum pores. Cells completely covered in porous tantalum surface, forming a mass overlap between cells and cells in vivo. Results: after 6 weeks of implantation, the boundary between material and bone tissue was clear, and a small amount of bone tissue crawled in porous tantalum pores. There were some trabeculae growing into the bone, the void and gap around the pores were visible in the unattached part, no tissue destruction and rejection were observed around the material, and 12 weeks after implantation, it was observed that the material and bone tissue grew well. Porous tantalum surfaces and pores in a large number of bone tissue into, material pores and tissue tight connection, a large number of bone trabeculae into, The results show that the metal tantalum coated scaffold prepared by atmospheric pressure chemical vapor deposition has good biocompatibility.
【作者单位】：大连理工大学电信学部生物医学工程系;大连大学附属中山医院骨科;
【基金】：国家十二五科技支撑项目(2012BAI17B02),项目名称:新一代骨科新型生物医用材料及产品研发国家十三五“国家重点研发计划”项目(2016YFC1102000),项目名称:生物活性脊柱及节段骨缺损修复器械的产品研发~~
【分类号】：R318.08

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