冷冻干燥牙本质对牙周膜干细胞影响的研究

发布时间：2018-05-28 09:16

本文选题：冷冻干燥牙本质(FDDM) + 牙周膜干细胞　；参考：《实用口腔医学杂志》2014年03期

【摘要】：目的:制作冷冻干燥牙本质生物支架(FDDM)并研究其对牙周膜干细胞(PDLSCs)的影响。方法:制作FDDM生物支架;将PDLSCs分别接种在FDDM、普通牙本质(D)、羟基磷灰石/磷酸三钙(HA)、盖玻片/α-MEM表面或浸提液中。MTT法检测细胞增殖;粘胶纤维红检测胶原分泌量;扫描电镜检测细胞形态;共聚焦显微镜观察各组细胞骨架形态。结果:研制出FDDM;MTT结果显示FDDM组细胞增殖活性高于HA组(P0.05);胶原检测显示FDDM组细胞分泌的胶原多于HA组(P0.05);电镜结果显示FDDM组细胞紧密粘附,细胞突起的数目和长度大于HA与盖玻片组;共聚焦结果显示,FDDM组细胞骨架较HA、盖玻片组粗大、清晰。结论:FDDM促进PDLSCs的增殖、胶原分泌,促进细胞粘附及细胞应力纤维形成。
[Abstract]:Aim: to prepare a freeze-dried dental scaffold (FDDM) and study its effect on periodontal ligament stem cells (PDLSCs). Methods: FDDM scaffolds were prepared, and PDLSCs was inoculated into FDDM, normal dentin, hydroxyapatite / tricalcium phosphate Hal, glass slide / 伪 -MEM surface or extractant, cell proliferation was detected by MTT method, collagen secretion was detected by viscose fiber red. The morphology of cytoskeleton was observed by scanning electron microscope and confocal microscope. Results: the results showed that the proliferative activity of FDDM group was higher than that of HA group, the collagen level of FDDM group was more than that of HA group, and the electron microscope showed that the cells in FDDM group were tightly adhered to each other, and the results of electron microscope showed that the cell proliferation activity of FDDM group was higher than that of HA group (P 0.05). The number and length of the cell processes were larger than those of HA and glass slide group, and the confocal results showed that the cytoskeleton of FDDM group was larger and clearer than that of HA group. ConclusionFDDM promotes PDLSCs proliferation, collagen secretion, cell adhesion and cell stress fiber formation.
【作者单位】：第四军医大学口腔医学院修复科;
【基金】：国家自然科学基金(编号:81271104,81200823)
【分类号】：R783.1

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