钙镁生物活性骨再生材料的研究

发布时间：2018-05-28 20:38

本文选题：缺钙羟基磷灰石 + 骨修复材料　；参考：《华东理工大学》2012年硕士论文

【摘要】：本课题包括镁替代磷酸钙骨水泥(MCPC)和介孔硅酸钙镁(OMC)两种钙镁基骨修复材料的研究。第一部分将活性氧化镁(MgO)与磷酸四钙(Ca4(PO4)2O),磷酸氢钙(CaHPO4)按照一定比例混合制备出新型的镁替代磷酸钙骨水泥。通过颗粒溶出的方法制备出MCPC多孔支架,支架材料在体外测试中表现出良好的生物降解性和细胞相容性。将MCPC多孔支架植入兔股骨缺损,支架表现出优良的生物相容性,随着植入时间的增加,材料逐步降解,12周时,材料完全降解,缺损区域被新骨所替代,材料显示出了优良的降解性和成骨能力。第二部分利用溶胶-凝胶法制备出了介孔硅酸钙镁材料,该材料具有规整的7nm左右的介孔孔道,比表面积高达1017 m2/go OMC在Tris-HCl溶液中具有良好的溶解性,在模拟体液中浸泡7天后,表面有大量磷灰石生成,显示出了优良的体外生物活性。将材料植入兔股骨缺损,随着植入时间的增长,新骨不断生成,OMC溃散成小的碎片,逐步降解,12周后,骨缺损基本被修复。对OMC的吸附与释放药物及大分子蛋白的性能进行了研究,结果表明,跟对照组的硅酸钙镁(CMS)相比,OMC材料能够吸附大量的药物以及一定量的大分子蛋白,且对吸附的药物／蛋白有明显的缓释效果。另外,对MCPC多孔支架和OMC的体内动物实验所获得的样本利用上海同步辐射光源进行生物医学成像研究,结果清晰直观的显示了材料逐步降解,新骨逐渐生成的过程。实验表明MCPC和OMC都是很有潜力的骨修复生物材料。
[Abstract]:In this paper, two kinds of calcium and magnesium based bone repair materials, MCPC (calcium phosphate cement) and OMC (mesoporous calcium silicate), are studied. In the first part, a new type of calcium phosphate cement was prepared by mixing active magnesium oxide (MgO) with tetracalcium phosphate (Ca4CO4PO4), calcium phosphate (CaHPO4) and calcium phosphate (CaHPO4) according to a certain proportion. MCPC porous scaffolds were prepared by particle dissolution method. The scaffolds showed good biodegradability and cytocompatibility in vitro. The MCPC porous scaffold was implanted into rabbit femur defect. The scaffold showed excellent biocompatibility. With the increase of implantation time, the material degraded completely at 12 weeks, and the defect area was replaced by new bone. The material showed excellent biodegradability and osteogenic ability. In the second part, mesoporous calcium silicate magnesium was prepared by sol-gel method. The material has regular mesoporous channels about 7nm, and its specific surface area is up to 1017 m2/go OMC. It has good solubility in Tris-HCl solution, and is soaked in simulated body fluid for 7 days. A large amount of apatite was formed on the surface, which showed excellent bioactivity in vitro. The material was implanted into the rabbit femur defect. With the increase of the implantation time, the new bone burst into small fragments. After 12 weeks of degradation, the bone defect was basically repaired. The adsorption and release of drugs and the properties of macromolecular proteins were studied. The results showed that the OMC materials could adsorb a large amount of drugs and a certain amount of macromolecular proteins compared with the control group. And the drug / protein adsorbed has obvious slow release effect. In addition, the biomedical imaging of MCPC porous scaffold and OMC in vivo animal experiment was studied by using Shanghai Synchrotron radiation source. The results clearly and directly showed the process of material degradation and the gradual formation of new bone. Both MCPC and OMC are potential biomaterials for bone repair.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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