新型纳米水凝胶接枝真丝缝合线抗菌性能研究

发布时间：2018-05-28 22:51

本文选题：真丝缝合线 + 纳米水凝胶　；参考：《东华大学》2016年硕士论文

【摘要】：医用真丝缝合线由于其稳定、易操作的特性成为了目前社会上广泛使用的缝合线之一。但是真丝缝合线作为一种不可吸收缝合线自身对细菌、病毒等没有抑菌作用,编织结构的缝隙以及丝线内部单纤维间隙为细菌提供隐匿场所,这些都增加了手术部位感染(SSIs)的概率。为了赋予真丝缝合线良好的抗菌性能,在一定范围内阻隔细菌与手术切口的接触,从而减少SSIs的发生概率。因此,研究具有抗菌性能的医用真丝缝合线具有重要的临床应用价值。本文采用新型生物可降解赖氨酸聚酯氨纳米水凝胶作为抗菌剂载体,基于常用医用高校抗菌剂的调查分析选择了抗菌剂三氯生,通过化学接枝的方法将纳米水凝胶接枝在真丝缝合线的表面。制备了能够防止外科手术感染(SSIs)的长效抗菌纳米水凝胶接枝真丝缝合线。另外,通过抗菌性能试验评价了载三氯生的纳米水凝胶接枝真丝缝合线(TLNGS)的抗菌性能。抗菌性能试验的前四天观察到TLNGS在琼脂培养基上具有稳定的抑菌圈。抗菌测试的结果表明接枝缝合线具有可以预防伤口早期感染的功能。与此同时,化学接枝处理后缝合线的物理机械功能受到的影响极小可以忽略不计。具有生物相容性以及生物降解功能的赖氨酸基水凝胶作为药物载体接枝在真丝缝合线表面并且提供了一种新的有效的通用抑菌性处理方式。制备载药水凝胶,通过酶降解赖氨酸水凝胶,自组装形成载药纳米水凝胶,通过对载药纳米水凝胶的抗菌功能评价,证实了水凝胶无抗菌功能以及抗菌剂三氯生的抗菌有效性;进一步研究了化学接枝后真丝缝合线的抗菌持久性,并与国外临床抗菌缝合线产品进行了抗菌持久性的对比研究;为了研究不同交联密度水凝胶对缝合线抗菌功能的影响,选择了两种不同交联密度水凝胶:水凝胶4-lys-4(1.5:1)以及4-lys-4(2:1),对缝合线进行接枝处理,制成了2种抗菌缝合线;对两种抗菌纳米水凝胶进行药物缓释,对接枝后的缝合线进行sem分析以及抗菌缓释性能测试,以及物理机械性能的相关评价。本课题得出的主要结论如下:水凝胶4-lys-4(1.5:1)、4-lys-4(2:1)采用胰蛋白酶生物降解7天后的纳米尺度分别约为60nm和90nm。两种水凝胶的粒径分布窄,有利于抗菌剂稳定的缓释速率;交联密度大的4-lys-4(2:1)载药量高;载药纳米水凝胶对金黄色葡萄球菌和大肠杆菌均具有优异的抗菌功能,未载药水凝胶对金黄色葡萄球菌以及大肠杆菌无抑菌功能;化学接枝后的真丝缝合线力学性能、缝合线的直径以及编织结构基本没有改变,符合标准。通过sem观察,与接枝前的真丝缝合线相比,抗菌真丝缝合线表面被均匀接枝了具有长效抑菌功能的抗菌纳米水凝胶粒子;两种接枝型抗菌缝合线具有优异的抗菌持久性,相同测试条件下与国外的临床用抗菌缝合线相比,具有更加持久的抗菌功能。且接枝交联密度越大的抗菌纳米水凝胶粒子的真丝缝合线的药物缓释抗菌功能越好。
[Abstract]:As a result of its stability and easy operation, medical silk suture has become one of the most widely used sutures in the society. However, the silk suture line, as an inabsorbable suture, has no bacteriostasis for bacteria, virus and so on. The probability of surgical site infection (SSIs) is increased. In order to give the good antibacterial properties of the silk suture line, the contact between the bacteria and the surgical incision is blocked in a certain range, thus reducing the probability of the occurrence of SSIs. Therefore, the study of the medical silk suture with antibacterial properties is of great importance in clinical application. The lysine polyester ammonia nano hydrogel is used as an antiseptic carrier. Based on the investigation and analysis of the antibacterial agents in medical colleges and universities, three chlorinated antibacterial agents are selected and the nano hydrogels are grafted onto the surface of the silk suture line by chemical grafting. The grafting of long effective antibacterial nanosels that can prevent the surgical infection (SSIs) is prepared. In addition, the antibacterial properties of three chlorinated nano hydrogels grafted with silk suture line (TLNGS) were evaluated through antibacterial properties test. The antibacterial properties of TLNGS were observed on the agar medium four days ago. The results of antimicrobial test showed that the branch suture could prevent early infection of the wound. At the same time, the physical and mechanical functions of the suture line after chemical grafting are negligible. The lysine based hydrogels with biocompatibility and biodegradability are grafted on the surface of the silk suture line as a drug carrier and provide a new and effective general antibacterial treatment method. Drug loading hydrogels were used to degrade lysine hydrogels by enzymes and self-assembled to form drug loaded nanosels. By evaluating the antibacterial function of the drug loaded nanosels, the antibacterial functions of the hydrogels and the antibacterial effectiveness of three chlorinated antimicrobial agents were confirmed, and the antibacterial durability of the silk suture line after chemical grafting was further studied, and it was associated with foreign presence. In order to study the antiseptic durability of the bed antibacterial suture products, two different crosslinked density hydrogels, hydrogels 4-lys-4 (1.5:1) and 4-lys-4 (2:1) were selected to study the effect of different crosslinking density hydrogels on the antibacterial function of the suture line, and 2 kinds of antibacterial suture lines were made and two kinds of antibacterial suture lines were made. The nano hydrogel carried out the drug release, the SEM analysis of the suture line after the grafting, the antibacterial release performance test, and the related evaluation of the physical and mechanical properties. The main conclusions are as follows: the hydrogel 4-lys-4 (1.5:1), 4-lys-4 (2:1) with trypsin degradation 7 days after the degradation of the nano scale of about 60NM and 90nm., respectively, two species The particle size distribution of the hydrogels is narrow, which is beneficial to the stable release rate of the antibacterial agent; the 4-lys-4 (2:1) with large crosslink density has high drug loading; the drug loaded nano hydrogel has excellent antibacterial function to Staphylococcus aureus and Escherichia coli, and the unloaded hydrogel has no bacteriostasis on Staphylococcus aureus and Escherichia coli. The mechanical properties of the silk suture, the diameter of the suture line and the knitting structure are basically unchanged, which conform to the standard. Compared with the silk suture before grafting, the surface of the antibacterial silk suture line is uniformly grafted with antibacterial nanoscale hydrogel particles with long effective bacteriostasis, and the two kinds of graft antibacterial suture lines have excellent antibacterial properties through SEM. Under the same test conditions, the antibacterial function of the antibacterial nano hydrogel particles with greater graft crosslinking density is better than that of the clinical antibacterial suture in foreign countries.
【学位授予单位】：东华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TQ427.26;R318.08

【共引文献】