纯钛表面薄层Nano-HA涂层的制备及生物学评价

发布时间：2018-05-29 18:54

本文选题：Nano-HA + 电化学沉积技术　；参考：《浙江大学》2012年硕士论文

【摘要】：目的：纳米级羟磷友石(Nano-hydroxyapatite, Nano-HA)与人体内羟磷灰石具有相似性,具有较好的生物学性能。电化学沉积技术可在多孔纯钛表面制备Nano-HA,且不破坏纯钛表面的多孔结构。本研究利用电化学沉积技术在多孔纯钛表面制备Nano-HA涂层,研究Nano-HA涂层对成骨细胞生物学活性的影响以及骨质疏松症种植体骨结合的影响。材料与方法：纯钛钛片和种植体经大颗粒金钢砂喷砂,HF/HNO3和HCl/H2SO4酸蚀。Nano-HA涂层制备采用两电极系统,以粗化钛片和种植体为阴极,铂片为阳极,400ml烧杯为电解槽,在含Ca(NO3)2(0.6mM)和NH4H2PO4(0.36mM)的200ml电解液中,在一定的温度和时间下,恒电压电沉积。Nano-HA涂层钛片和种植体作为实验组,多孔表面钛片和种植体作为对照组。场发射扫描电镜(FSEM)、X射线衍射仪(XRD)、红外光谱仪(FTIR)分析涂层表面结构,表面粗糙度测试仪(TR240)检测表面粗糙度。实验一：将小鼠前成骨细胞(MC3T3-E1)接种于上述两组钛片上,测定细胞总蛋白、碱性磷酸酶(Alkaline phosphatase, ALP)、骨钙素(Osteocalcin).实验二：建立大鼠骨质疏松症动物模型,将对照组和实验组种植体植入大鼠两侧胫骨远端,术后4周和12周处死动物,制作硬组织切片,采用亚甲基蓝-碱性品红法染色,对种植体-骨接触率和种植体螺纹内骨密度进行测量；采用便携式数字扭力测试仪(BS30)观察分析种植体和骨组织的结合力,并对两组种植体的扭力值进行统计学分析。结果：钛片表面经喷砂-双重酸蚀处理后形成多级微孔、波浪状的形貌；电化学沉积技术在钛片表面制备截面为规则六边形的纳米级晶体。XRD和FTIR分析表明该涂层是标准的HA。对照组和HA涂层组的表面粗糙度(Ra)平均值分别为1.185±0.061和1.167±0.096。细胞实验的结果显示HA组能促进MC3T3-E1细胞增殖,同时也能增强ALP活性和OC分泌。动物实验统计学表明,术后4周和12周Nano-HA涂层种植体明显增加骨质疏松症大鼠种植体与新骨接触率和种植体螺纹内骨密度(P0.05),增加种植体与新骨之间的结合力(P0.05)。结论：本实验证实该电化学沉积技术制备的Nano-HA涂层可促进成骨细胞的生物学活性以及骨质疏松症种植体骨结合。
[Abstract]:Objective: Nano-hydroxyapatite (Nano-HA) is similar to human hydroxyapatite and has good biological properties. Nano-HA can be prepared on porous titanium surface by electrochemical deposition without destroying the porous structure of pure titanium surface. In this study, Nano-HA coatings were prepared on porous pure titanium by electrochemical deposition technique. The effects of Nano-HA coating on the biological activity of osteoblasts and the osseous bonding of osteoporosis implants were studied. Materials and methods: Pure titanium sheets and implants were coated with large grain au / HNO _ 3 and HCl/H2SO4 acid etching. Nano-HA was prepared by using a two-electrode system, in which roughened titanium plates and implants were used as cathode, platinum plates as anode and 400ml beaker as electrolyzer, in 200ml electrolyte containing Cagno _ 3N _ 3N _ 2O _ 2O _ 20.6mm) and NH _ 4H _ 2PO _ 4O _ 40.36mM). Nano-HA coated titanium plates and implants were used as experimental group and porous surface titanium plates and implants as control group at certain temperature and time. The surface structure of the coating was analyzed by FTIR, and the surface roughness was measured by the surface roughness tester TR240). Experiment 1: the mouse preosteoblast (MC3T3-E1) was inoculated on the above two groups of titanium tablets, and the total cell protein, alkaline phosphatase (ALP), osteocalcinine (Osteocalcinia) were determined. Experiment 2: the animal model of osteoporosis in rats was established. The implants of the control group and the experimental group were implanted into the distal tibia of the rats on both sides. The animals were killed 4 and 12 weeks after operation, and the hard tissue sections were made and stained by methylene blue alkaline fuchsin method. The implant-bone contact rate and bone mineral density in implant thread were measured, and the adhesive force of implant and bone tissue was observed and analyzed by means of portable digital torsion tester (BS30), and the torque values of two groups of implants were analyzed statistically. Results: After sandblasting and double acid etching, multistage micropores and wavy morphology were formed on the surface of titanium sheet, and nanocrystalline crystals with regular hexagonal cross section were prepared by electrochemical deposition technique. XRD and FTIR analysis showed that the coating was a standard HAA coating. The average values of surface roughness in the control group and HA coated group were 1.185 卤0.061 and 1.167 卤0.096, respectively. The results of cell experiment showed that HA could promote the proliferation of MC3T3-E1 cells, but also enhance the activity of ALP and the secretion of OC. Animal statistics showed that Nano-HA coated implants significantly increased the contact rate between implant and new bone and bone mineral density (P0.05) in implant thread, and increased the binding force between implant and new bone at 4 and 12 weeks postoperatively in rats with osteoporosis. Conclusion: The results showed that the Nano-HA coating prepared by the electrochemical deposition technique could promote the biological activity of osteoblasts and the osseointegration of osteoporosis implants.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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