肽核酸芯片光导原位合成系统中微流体反应器的研制

发布时间：2018-05-31 09:06

本文选题：肽核酸芯片 + 光导原位合成　；参考：《中南大学》2012年硕士论文

【摘要】：肽核酸作为DNA的类似物,在与相应的DNA或RNA序列杂交反应时,表现出更高的特异性和更好的稳定性。因此由肽核酸单体合成的肽核酸芯片作为一种新型的基因芯片,近年来在各种基因研究中得到了广泛的应用。在肽核酸芯片的几种合成方法中,光导原位合成技术以其合成速度快、芯片密度高、成本低等优点表现出全新的竞争力。而微流体反应器作为光导原位合成的场所,其性能好坏直接影响合成的效果。本文按照反应器在流速均匀性、放置方位无限制性、密封性、化学稳定性等方面的要求,从反应器结构和反应池形状两方面设计了一种新的微流体反应器。该反应器采用压装式结构,主要由基座、反应池板、玻片、橡胶圈和旋盖组成。多种定位和固定设计使反应器的组装简单,使用方便。利用PDMS制作的反应池板上设有一个依据仿生学的原理设计的类似梭形的反应池。为了从理论上证明梭形反应池的流场分布均匀效果好于常规的菱形反应池,建立了张角为125度、135度和145度情况下的几组模型,利用Fluent计算流体力学软件进行仿真后,通过对仿真数据的分析,显示了梭形反应池的均匀率R更大,均匀效果更好。进一步增大张角和改变进出口宽度进行仿真后,优化了梭形反应池的设计。将反应器按照最终设计方案加工装配后,经过手工实验并用于光导原位合成系统中进行实际的曝光反应,最终得到了均一的与掩模一致的图案,验证了本设计的有效性,可以广泛应用于各种基因芯片的合成系统中。
[Abstract]:As an analogue of DNA, peptide nucleic acid exhibits higher specificity and better stability when hybridized with corresponding DNA or RNA sequences. Therefore, peptide nucleic acid chips synthesized from peptide nucleic acid monomers as a new type of gene chip, have been widely used in various gene research in recent years. Among several synthesis methods of peptide nucleic acid chips, photoconductive in situ synthesis technology has the advantages of high synthesis speed, high chip density and low cost. The performance of microfluidic reactor, as the site of photoconductive in situ synthesis, directly affects the effect of synthesis. A new micro-fluid reactor was designed in this paper according to the requirements of the reactor in terms of the uniformity of the flow rate, the location of the reactor, the sealing and the chemical stability of the reactor, in terms of the structure of the reactor and the shape of the reactor cell. The reactor consists of a base, a reactor plate, a glass slide, a rubber ring and a cover. A variety of positioning and fixation designs make the reactor assembly simple and easy to use. There is a fusiform reaction cell designed according to the principle of bionics on the plate of reaction cell made by PDMS. In order to prove theoretically that the uniform distribution of flow field of fusiform reaction cell is better than that of conventional rhombic reactor, several models with tension angle of 125 degrees or 135 degrees and 145 degrees are established, and the simulation results are carried out by using Fluent computational fluid dynamics software. Through the analysis of the simulation data, it is shown that the uniform rate R of the fusiform reaction cell is larger and the uniformity effect is better. After further increasing the opening angle and changing the width of inlet and outlet, the design of fusiform reaction cell is optimized. After the reactor was processed and assembled according to the final design scheme, after manual experiments and used in the photoconductive in-situ synthesis system for the actual exposure reaction, a uniform pattern consistent with the mask was finally obtained, and the effectiveness of the design was verified. It can be widely used in the synthesis system of various gene chips.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.6

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