超声对纳米多聚体靶向释放DNA影响的实验性研究

发布时间：2018-05-31 10:15

本文选题：超声降解 + 纳米多聚体　；参考：《中国生化药物杂志》2014年01期

【摘要】：目的通过超声照射纳米多聚体实验,了解超声对纳米多聚体靶向可控释放DNA的影响。方法实验用溶剂蒸干法制备纳米多聚体—聚乳酸/乙醇酸共聚物(Polylactic/poly glycolic acld,PLGA),为表面携带雄激素受体PLGA纳米颗粒,其包裹有编码荧光蛋白(GFP)的DNA。配制PLGA溶液2 h后,用2种超声方式,不同占空比及不同时间辐照后将溶液离心,定量观察DNA释放情况及细胞荧光表达效果。结果超声能破坏PLGA纳米壳,并释放DNA。辐照组的多聚体DNA释放量均高于对照组;DNA释放量随超声辐照的声强、时间增加变化不显著;连续超声波辐照降解作用略强于脉冲波;胰淀粉酶对DNA释放影响不大。结论体外实验证实超声有促进PLGA纳米多聚体降解及DNA释放的作用。
[Abstract]:Objective to investigate the effects of ultrasound irradiation on the controlled release of DNA in nanomaterials. Methods Polylactic-polylactic acid / glycolic acid copolymers were prepared by solvent drying method. Polylactic / poly glycolic acldPLGAA nanoparticles were prepared on the surface of PLGA nanoparticles, which were encapsulated with fluorescent protein (GFP). After 2 hours of preparation of PLGA solution, the solution was centrifuged with two ultrasonic methods, different duty cycle and different time. The release of DNA and the effect of fluorescence expression were observed quantitatively. Results Ultrasound could destroy PLGA nanoshell and release DNA. The amount of DNA released by irradiation group was higher than that of control group, and the time increased with the sound intensity of ultrasound irradiation. The degradation effect of continuous ultrasound irradiation was slightly stronger than that of pulse wave, and the effect of amylase on DNA release was not significant. Conclusion Ultrasound can promote the degradation of PLGA nanopolymer and the release of DNA in vitro.
【作者单位】：深圳市人民医院;暨南大学第二临床医学院超声科;香港大学深圳医院放射科;暨南大学第二临床医学院内科;珠海市卫生局科技处;湖北省中山医院外科;美国堪萨斯大学医学中心;
【基金】：深圳市战略性新兴产业发展专项(JCYJ20130402101926971) 深圳市科技创新二批基础项目(JCYJ 20120829170525005) 深圳市科技创新国家和省科研项目配套计划(ZYC 201105170257A) 国家自然科学基金面上项目(81270857)
【分类号】：R318.08

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