壳聚糖—凡士林纱布的制备及其对创面愈合的作用研究

发布时间：2018-06-02 02:18

本文选题：壳聚糖 + 敷料　；参考：《浙江大学》2017年硕士论文

【摘要】：目的制备功能性的壳聚糖新型纱布——壳聚糖-凡士林纱布(Chitosan-Vaseline gauze,CVG),并检测其细胞毒性、抑菌性、保湿性,及研究其对大鼠背部创面愈合的作用。方法以等质量的高低分子壳聚糖,凡士林,石蜡油,无菌纱布为原料,将无菌纱布充分浸渍于高低分子壳聚糖混合液,制成壳聚糖纱布;配置凡士林石蜡油混合液,以凡士林石蜡油混合液包裹壳聚糖纱布,制备壳聚糖-凡士林纱布。借助扫描电镜(Scanningelectron microscopy,SEM),更客观更直观的来观察壳聚糖-凡士林纱布的镜下结构及红外光谱法对其进行光谱分析,验证壳聚糖是否成功的融入了凡士林纱布(Vaseline gauze,VG)中。采用CCK-8(Cell Counting Kit-8)法检测其细胞毒性;采用抑菌圈法检测其抑菌性;采用数字皮肤水分检测仪检测其保湿性。将大鼠随机分为实验组和对照组,按照外科方法建立大鼠背部创面模型。实验组覆盖壳聚糖-凡士林纱布,对照组覆盖凡士林纱布。术后3、7、14天,测量创面面积,获取皮肤修复组织。观察大鼠创面面积变化情况直至所有大鼠创面达到愈合状态。制作切片行HE(Hematoxylin-eosin),CD31免疫组织化学和Masson染色,多方面评估两组大鼠创面愈合情况。结果在我们制备的壳聚糖-凡士林纱布中,通过扫描电镜及红外光谱分析,基本证明壳聚糖成功地融入了我们的纱布。而且,通过一系列的测试实验,可以证明,相比于凡士林纱布,壳聚糖-凡士林纱布无明显的细胞毒性,能显著抑制金黄色葡萄球菌和大肠杆菌的生长,并且具有更好的保湿作用。在术后3、7、14天,壳聚糖-凡士林纱布组创面面积均明显小于凡士林纱布对照组(p0.05),创面上皮化时间明显缩短(14.83 ±0.75天vs 18.50 ±0.55天,p =0.00),新生血管出现更丰富和更早成熟化,胶原增生更加有序整齐,接近于正常皮肤组织中的胶原生长。结论相比于凡士林纱布,壳聚糖-凡士林纱布无明显细胞毒性,具有更好的抑菌性和保湿性。而且在大鼠创面愈合实验中,肉眼观察和组织病理学结果均证明,壳聚糖-凡士林纱布较凡士林纱布能明显的促进创面愈合。
[Abstract]:Objective to prepare a new type of chitosan gauze, Chitosan-Vaseline gauze CVGG, and to detect its cytotoxicity, bacteriostasis, moisture retention and its effect on the healing of rat back wound. Methods chitosan gauze was prepared by impregnating aseptic gauze with the mixture of high and low molecular chitosan, vaseline, paraffin oil and aseptic gauze. Chitosan gauze was prepared by encapsulating chitosan gauze with Vaseline paraffin oil mixture. By means of scanning electron microscope (SEM) and scanning electron microscope (Scanningelectron microscopyn), the structure of chitosan-vaseline gauze was observed more objectively and intuitively. The results showed that chitosan was successfully integrated into Vaseline gauze (VG). The cytotoxicity was detected by CCK-8(Cell Counting Kit-8 method, the bacteriostasis was detected by bacteriostasis method, and the moisture retention was detected by digital skin moisture detector. Rats were randomly divided into experimental group and control group. The experimental group was covered with chitosan-vaseline gauze and the control group covered with vaseline gauze. The wound area was measured and skin repair tissue was obtained at 3 ~ 7 ~ 14 days postoperatively. The changes of wound area in rats were observed until all the wounds reached the state of healing. The wound healing of the two groups was evaluated by immunohistochemistry and Masson staining. Results in our chitosan-vaseline gauze, it was proved by SEM and IR analysis that chitosan was successfully integrated into our gauze. Moreover, through a series of test experiments, it can be proved that chitosan vaseline gauze has no obvious cytotoxicity compared with vaseline gauze, and can significantly inhibit the growth of Staphylococcus aureus and Escherichia coli. And has better moisturizing effect. The wound area in the chitosan vaseline gauze group was significantly smaller than that in the vaseline gauze control group (P 0.05), and the wound epithelization time was significantly shortened by 14.83 卤0.75 days vs 18.50 卤0.55 days, and the neovascularization appeared richer and earlier maturation. Collagen proliferation is more orderly, close to the growth of collagen in normal skin tissue. Conclusion compared with vaseline gauze, chitosan-vaseline gauze has no obvious cytotoxicity and has better bacteriostatic and moisturizing properties. The results of naked eye observation and histopathology showed that chitosan-vaseline gauze could obviously promote wound healing compared with vaseline gauze.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

【相似文献】