MG63细胞与国产多孔钽材料共培养后成骨相关因子的表达研究

发布时间：2018-06-03 18:07

本文选题：国产多孔钽 + MG细胞　；参考：《中国修复重建外科杂志》2014年11期

【摘要】：目的观察MG63细胞与国产多孔钽材料共培养后成骨相关因子的表达,探讨该材料作为骨组织工程支架的可行性。方法实验分为3组,A组为多孔钽材料与MG63细胞共培养组,B组为多孔钽浸提液与MG63细胞共培养组,C组为单纯MG63细胞培养组。A组于培养后3、5、7 d行扫描电镜观察细胞在材料上的黏附情况;培养5 d分别采用免疫组织化学染色和Western blot检测各组成骨相关因子Runt相关转录因子2(Runt-related transcription factor2,Runx-2)、骨钙素(osteocalcin,OC)和纤维连接蛋白(fi bronectin,FN)的表达。结果培养3 d,A组细胞在材料表面及孔隙内黏附生长,细胞排列较稀疏,突起少;5 d,相邻细胞互相连接成片状,覆盖材料表面及孔隙内壁;7 d,细胞分泌大量细胞外基质,覆盖大部分材料表面。各组细胞均有不同程度Runx-2、OC和FN表达,其中A组细胞质深染,表达较其余2组明显。免疫组织化学染色和Western blot定量分析示,A组Runx-2、OC表达量显著高于B、C组(P0.05);B、C组间差异无统计学意义(P0.05)。3组间FN表达量比较,差异均无统计学意义(P0.05)。结论国产多孔钽材料有利于MG63细胞的早期黏附、生长,并可能影响Runx-2、OC的表达,可用作骨组织工程支架材料。
[Abstract]:Objective to observe the expression of osteoblast-related factors in MG63 cells co-cultured with porous tantalum and to explore the feasibility of using the material as scaffold for bone tissue engineering. Methods the experiment was divided into 3 groups: group A: porous tantalum and MG63 cell coculture group B: porous tantalum extract and MG63 cell coculture group C group: simple MG63 cell culture group. Group A was observed by scanning electron microscope on the 7th day after 3 days of culture. Adherence to materials; The expression of 2(Runt-related transcription factor2 Runt related transcription factors (2(Runt-related transcription factor2C), osteocalcin (OC) and fibronectin (FN) were detected by immunohistochemical staining and Western blot on the 5th day of culture. Results the cells in group A were adhered and grown on the surface and pore of the material for 3 days. The cells were arranged sparsely, the processes were less than 5 days, the adjacent cells were connected to each other into flakes, covering the surface of the material and the inner wall of the pore for 7 days, the cells secreted a large amount of extracellular matrix. Cover the surface of most materials. The expression of Runx-2OC and FN in group A was significantly higher than that in group A. Immunohistochemical staining and Western blot quantitative analysis showed that the expression of Runx-2oc in group A was significantly higher than that in group B (P 0.05). There was no significant difference in the expression of FN between group A and group B (P 0.05), and there was no significant difference in the expression of FN between group A and group B (P 0.05). Conclusion porous tantalum made in China is beneficial to the early adhesion and growth of MG63 cells and may affect the expression of Runx-2OC. it can be used as scaffold material for bone tissue engineering.
【作者单位】：南方医科大学研究生学院;河北联合大学基础医学院人体解剖系;唐山市第二医院关节一科;河北联合大学实验中心;河北联合大学附属医院骨科;唐山市第二医院手一科;
【基金】：国家科技部科技支撑课题资助项目(2012BAE06B03)~~
【分类号】：R318.08

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