两种方法处理后钛合金表面的生物矿化及其性能研究

发布时间：2018-06-06 05:01

本文选题：钛合金 + 酸碱处理　；参考：《兰州大学》2012年硕士论文

【摘要】：钛合金具有理想的理化性能和良好的生物相容性,是临床常用的人工植入材料,由于其本身没有骨诱导活性,与周围骨组织不能形成足够的化学结合,使临床应用受限。羟基磷灰石(Hydroxyapatite, HA)与骨组织的化学成分和结构类似,可通过化学键与骨组织结合并诱导新骨生成。因此,采用羟基磷灰石涂层方法已成为钛合金表面常用的改性手段之一,但是涂层与基底结合存在一定问题,影响了植入体长期的稳定性,有必要探讨新的方法对钛合金表面进行改性。本论文采用两种化学方法：酸碱结合法和多巴胺自组装技术对钛合金表面进行处理形成过渡层,通过模拟体液(SBF)仿生矿化制备羟基磷灰石活性涂层。利用扫描电子显微镜(SEM)、X射线衍射仪(XRD)、光学接触角测量仪、傅里叶变换红外光谱仪(FTIR)、X-射线光电子能谱仪(XPS)等分析测试手段对改性前、后钛合金表面的形貌、物相结构、润湿性以及组成成分等进行了分析表征。同时,采用材料与成骨细胞体外共培养的方式,研究了两种方法诱导生成的HA活性涂层的细胞相容性。主要研究结果如下： 1)通过对不同酸溶液体系及不同浓度的碱液处理钛合金表面的方法进行对比,观察到不同溶液处理后的钛合金表面腐蚀状况不同,单一的处理不如酸碱联合处理得到的网孔状结构均匀。借助SEM、XRD和FTIR等分析,发现酸碱结合处理后仿生矿化形成了成分单一的HA涂层。细胞实验表明成骨细胞早期粘附数量及增殖率高于未处理的对照组,显示了良好的细胞相容性。 2)通过分子自组装技术在钛合金表面自组装聚多巴胺形成过渡层,经生物矿化形成HA活性涂层,通过SEM、XRD与FTIR表征证实所得涂层为含碳的羟基磷灰石,且排列比较致密。细胞实验也证实了该种方法得到的HA涂层表现出了优异的细胞粘附与增殖能力,有望成为钛合金表面活化处理的一种新的方法,具有良好的临床应用前景。
[Abstract]:Titanium alloy has ideal physical and chemical properties and good biocompatibility. It is a commonly used artificial implant material in clinic. Because of its lack of bone inductive activity, it can not form enough chemical combination with surrounding bone tissue, so its clinical application is limited. Hydroxyapatite (HA), which is similar to the chemical composition and structure of bone tissue, can bind to bone tissue and induce new bone formation by chemical bond. Therefore, hydroxyapatite coating method has become one of the commonly used modification methods on titanium alloy surface, but there are some problems in the combination of coating and substrate, which affect the long-term stability of the implant. It is necessary to explore a new method for surface modification of titanium alloy. In this paper, two chemical methods, acid-base binding method and dopamine self-assembly technique, were used to treat titanium alloy surface to form transition layer. Hydroxyapatite active coating was prepared by simulated SBF biomimetic mineralization. The surface morphology and phase structure of titanium alloy before and after modification were analyzed by means of scanning electron microscope (SEM), SEMU X-ray diffractometer, optical contact angle measuring instrument, Fourier transform infrared spectrometer (FTIR) and X-ray photoelectron spectroscopy (XPS). The wettability and composition were analyzed and characterized. At the same time, the cytocompatibility of HA active coatings induced by two methods was studied by co-culture of materials and osteoblasts in vitro. The main findings are as follows: 1) by comparing the methods of treating titanium alloy surface with different acid solution system and different concentration of alkali solution, it is observed that the corrosion condition of titanium alloy treated with different solution is different. The single treatment is not as uniform as the mesh structure obtained by acid-base combined treatment. By means of FTIR and FTIR analysis, it was found that the HA coating with single composition was formed by biomimetic mineralization after acid-base treatment. Cell experiments showed that the number and proliferation rate of osteoblasts in the early stage were higher than those in the untreated control group and showed good cytocompatibility. 2) Self-assembled polydopamine transition layer was formed on the surface of titanium alloy by molecular self-assembly technique. HA active coating was formed by biomineralization. The coating was characterized by FTIR and FTIR, and the coating was carbon containing hydroxyapatite with compact arrangement. The cell experiments also confirmed that the HA coating obtained by this method showed excellent cell adhesion and proliferation, which is expected to be a new method for surface activation treatment of titanium alloy, and has a good prospect of clinical application.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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