灌注法制备全肾脏脱细胞基质支架的体内生物相容性

发布时间：2018-06-09 04:05

本文选题：组织工程 + 肾　；参考：《中国组织工程研究》2015年16期

【摘要】：背景:应用灌注法制备的大鼠全肾脏脱细胞基质支架具有良好的体外细胞相容性,但其体内生物相容性尚不明确。目的:应用灌注法制备大鼠全肾脏脱细胞基质支架,检测其体内生物相容性。方法:应用灌注法制备Wistar大鼠全肾脏脱细胞基质支架,进行以下实验:1急性毒性实验:在小鼠腹腔分别注射全肾脏脱细胞基质支架浸提液、生理盐水及苯酚。2溶血实验:将抗凝新西兰兔血分别与全肾脏脱细胞基质支架浸提液、生理盐水及蒸馏水混合。3热源实验:向新西兰兔耳缘静脉注射全肾脏脱细胞基质支架浸提液。4内皮刺激实验:在新西兰兔皮下注射全肾脏脱细胞基质支架浸提液,观察有无皮肤刺激反应。5皮下植入实验:将全肾脏脱细胞基质支架埋入新西兰兔背部皮下。结果与结论:全灌注法制备的Wistar大鼠全肾脏脱细胞基质支架无细胞残留,未引起全身毒性反应、急性溶血反应、热源反应及皮肤刺激反应,植入兔体内具有良好的组织相容性。说明大鼠全肾脏脱细胞基质材料在动物体内具有很好的生物相容性。
[Abstract]:Background: the acellular matrix scaffolds prepared by perfusion method have good cytocompatibility in vitro, but their biocompatibility in vivo is unclear. Aim: to study the biocompatibility of acellular matrix scaffolds in rat kidney by perfusion method. Methods: the acellular matrix scaffolds of Wistar rats were prepared by perfusion method. The following acute toxicity experiments were carried out: the whole kidney acellular matrix scaffolds were injected intraperitoneally into mice, and the extractions of acellular matrix scaffolds were injected into mice intraperitoneally. Physiological saline and phenol .2 hemolysis test: the anticoagulant New Zealand rabbit blood and the whole kidney acellular matrix scaffold extraction solution, Experiment of mixed .3 heat source with normal saline and distilled water: the whole kidney acellular matrix stent extract was injected into the ear of New Zealand rabbit into vein, and the endothelium stimulation experiment was done: the whole kidney acellular matrix stent extract was injected subcutaneously into New Zealand rabbit, and the whole kidney acellular matrix stent extract was injected subcutaneously into New Zealand rabbit. To observe whether there was skin irritation, 5. 5 subcutaneous implantation experiment: the whole kidney acellular matrix scaffold was implanted subcutaneously into the back of New Zealand rabbit. Results and conclusion: the acellular matrix scaffolds of Wistar rats prepared by the method of total perfusion had no cell residues, no systemic toxicity, acute hemolysis, heat source reaction and skin irritation, and had good histocompatibility in rabbits. The results showed that the acellular matrix material of rat kidney had good biocompatibility in vivo.
【作者单位】：汕头大学医学院第二附属医院泌尿外科;南方医科大学珠江医院泌尿外科;
【基金】：广东省医学科学技术研究基金(A2012394)~~
【分类号】：R318.08

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