应用同位素稀释法验证生物电阻抗分析法测定人体成分的研究

发布时间：2018-06-10 00:42

本文选题：生物电阻抗分析法 + 同位素稀释法　；参考：《肠外与肠内营养》2014年03期

【摘要】：目的:验证生物电阻抗分析法(BIA)用于成人体成分分析的精度。方法:以同位素稀释法技术作为标准方法。纳入健康受试者,空腹留取基础尿液。应用BIA对每名受试者进行人体成分测定,然后口服双标水(2H和18O),经5 h在体内平衡后再次留取标本。根据标本中同位素变化量计算出总体水(TBW),进而通过公式计算得出去脂瘦体组织(FFM)、脂肪组织(FM)以及FM%。比较两种方法测得结果,建立BIA校正线性方程。结果:纳入12名健康受试者,体重指数(BMI)为21.53±1.51。2H和18O测定人体各成分间无统计学差异,选用2H作为校正BIA的标准方法。BIA测得TBW、FFM、FM和FM%与2H测得结果相关系数分别为0.959,0.959,0.563,0.780。TBW校正方程为:y=4.057+0.798x。结论:BIA测定人体成分与标准同位素稀释法有等效性,结果稳定,相关性好,适于快速评价人群的TBW和FFM等人体成分。
[Abstract]:Objective: to verify the accuracy of bioelectric impedance analysis (BIA) in the analysis of human components. Methods: isotope dilution technique was used as the standard method. Basic urine was taken from healthy subjects on an empty stomach. BIA was used to determine the composition of each human body, and then the double labeled water 2H and 18OX were administered orally. After 5 hours of equilibrium, the specimens were collected again. According to the isotopic changes in the sample, the total TBWN was calculated, and the FFMI (FFMF) and FM50 (FFM) and FM50 were obtained from the formula. The BIA corrected linear equations are established by comparing the results obtained by two methods. Results: the body mass index (BMI) was 21.53 卤1.51.2H and 18O respectively. There was no statistical difference between the body mass index (BMI) and 18O. The correlation coefficients between TBWFFMFM and FM% and 2H were 0.9590.9595 90.563H 0.780.TBW correction equation was: yyyyyang4.057 0.798x.BIA was used as the standard method to calibrate BIA, and the correlation coefficients of TBWFFMFM and FM% with 2H were 0.9590.950.563and 0.780.TBW, respectively. Conclusion the determination of human components by BIA is equivalent to that of standard isotope dilution method. The results are stable and have good correlation. It is suitable for rapid evaluation of human components such as TBW and FFM.
【作者单位】：河北北方学院;北京协和医院肠外肠内营养科;清华大学体育部;中国科学院地质与地球物理研究所稳定同位素实验室;
【分类号】：O657.1;R444

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