聚甲基丙烯酸甲酯静电纺丝纳米纤维对大鼠原代星形胶质细胞生长的影响

发布时间：2018-06-10 01:03

本文选题：静电纺丝 + 聚甲基丙烯酸甲酯　；参考：《南方医科大学学报》2014年11期

【摘要】：目的探讨聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)电纺纳米纤维的拓扑线索对于大鼠原代星形胶质细胞生长能力及方式的影响,为脊髓损伤后植入性细胞支架的构建提供前期基础。方法构建具有随机或有序拓扑结构的PMMA电纺纳米纤维;分离并纯化大鼠原代星形胶质细胞;利用PMMA薄膜作为对照,利用慢病毒技术转染绿色荧光蛋白基因作为显色手段,分析在星形胶质细胞不同拓扑结构纤维支架上的生长特点。结果随机及有序PMMA电纺纤维均能支持星形胶质细胞的生长,其拓扑结构能够显著影响星形胶质细胞的生长方式,在有序纤维系统上细胞突起的生长方向能够和基质纤维的延伸方向保持高度一致;通过绿色荧光蛋白和基质纤维的合成图,发现在两种拓扑结构的纤维系统上,细胞突起均能依附在纤维上向远处延伸;较之PMMA薄膜,在有序纤维上的星形胶质细胞能生成更长的细胞突起(P0.01),而在随机纤维上的细胞则形成更短的突起(P0.01)。结论 PMMA电纺纳米纤维的拓扑结构能够显著影响大鼠原代星形胶质细胞生长能力及方式,其作为植入性支架具有减轻脊髓损伤后损伤灶胶质瘢痕形成的潜在价值。
[Abstract]:Objective to investigate the effect of topological clues of polymethylmethacrylate (PMMA) electrospun nanofibers on the growth ability and manner of primary astrocytes in rats, and to provide a basis for the construction of scaffolds after spinal cord injury. Methods PMMA electrospun nanofibers with random or ordered topologies were constructed, primary astrocytes were isolated and purified. The growth characteristics of astrocytes on different topological fiber scaffolds were analyzed. Results both random and ordered PMMA electrospun fibers could support the growth of astrocytes, and their topological structure could significantly affect the growth pattern of astrocytes. In the ordered fiber system, the growth direction of the cell processes can be highly consistent with the extension direction of the matrix fiber, and through the green fluorescent protein and matrix fiber synthesis diagram, it is found that in the two kinds of topological fiber system, the growth direction of the cell protuberance can be highly consistent with the extension direction of the matrix fiber. Compared with PMMA membrane, astrocytes on the ordered fibers can produce longer cellular processes P0.01N, while those on random fibers can form shorter processes P0.01. Conclusion the topological structure of PMMA electrospun nanofibers can significantly affect the growth ability and mode of primary astrocytes in rats, and it has the potential value of reducing the formation of focal glial scar after spinal cord injury as an implanted scaffold.
【作者单位】：重庆医科大学附属第一医院神经外科;重庆医科大学药学院药理教研室//重庆市生物化学与分子药理学重点实验室;
【基金】：国家自然科学基金资助项目(81100906) 国家临床重点专科建设项目(财社[2011]170号) 重庆市科委前沿与应用基础研究计划资助项目(cstc2014jcyjA10061)~~
【分类号】：R318.08

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