负载ZnO QDs的多功能胶原基复合水凝胶作为角膜替代物的研究

发布时间：2018-06-18 14:01

本文选题：胶原 + 互穿聚合物互穿网络　；参考：《天津大学》2012年硕士论文

【摘要】：一种胶原/聚2-丙烯酰胺-2-甲基-1-丙磺酸酸钠(PNaAMPS)互穿聚合物网络(IPN)水凝胶由交联剂碳二亚胺(EDC)/N-羟基琥珀酰亚胺(NHS)交联猪皮I型胶原,以聚乙二醇二丙烯酸酯(PEGDA)交联NaAMPS同步聚合制备。将冷冻干燥后的IPN凝胶浸泡在ZnO量子点水溶液中得到负载有ZnO量子点的胶原基复合水凝胶。对凝胶各种性能进行表征,IPN凝胶的平衡含水量和白光透过率都在90 %左右,PNaAMPS网络的引入提高了IPN水凝胶的力学强度,ZnO量子点的引入未对凝胶物理性能造成明显影响。ZnO量子点在凝胶中起双重作用:通过对ZnO量子点荧光的观测可以实时跟踪凝胶的降解情况;作为胶原酶的抑制剂提高了凝胶在胶原酶溶液中的稳定性。复合凝胶体系中的聚阴离子网络PNaAMPS可以有效地平衡由于引入带正电的ZnO量子点所带来的毒性,兔角膜前基质成纤维细胞(RCFBF)在其表面的生长情况与纯胶原凝胶相当,表明体系的细胞毒性较低,生物相容性良好。以此复合凝胶为培养细胞,由商业化转染试剂PolyJetTM所介导的基因转染实验表明,荧光素酶和加强型绿色荧光蛋白都可以在RCFBF细胞中得到有效地表达。此负载有ZnO量子点的胶原基复合水凝胶可实现对降解过程进行跟踪、抑制,对角膜细胞具有良好的生物相容性,亦可通过在其表面生长的细胞达到基因递送的目的,是一种潜在的组织工程角膜材料。
[Abstract]:A collagen / polyacrylamide sodium 2-methyl-1-propylsulfonate (PNaAMPS) interpenetrating polymer network (IPN) hydrogel crosslinked pig skin type I collagen by crosslinking agent carbodiimide EDCN / N-hydroxysuccinimide (NHS). Simultaneous polymerization of polyethylene glycol diacrylate (PEGDA) crosslinked NaamPS was carried out. The freeze dried IPN gel was immersed in ZnO quantum dot aqueous solution to obtain collagen based composite hydrogels loaded with ZnO quantum dots. The equilibrium water content and white light transmittance of IPN gel are all about 90%. The introduction of PNaAMPS network improves the mechanical strength of IPN hydrogel and the introduction of ZnO quantum dot has no obvious effect on the physical properties of IPN gel. Ring. ZnO quantum dots play a dual role in the gel: the degradation of the gel can be tracked in real time by observing the fluorescence of ZnO quantum dots; As an inhibitor of collagenase, the stability of gel in collagenase solution was improved. The polyanion network PNaAMPS in the composite gel system can effectively balance the toxicity caused by the introduction of positively charged ZnO quantum dots, and the growth of rabbit anterior stromal fibroblast (RCFBF) on its surface is similar to that of pure collagen gel. The results showed that the cytotoxicity of the system was low and the biocompatibility was good. The gene transfection mediated by the commercial transfection reagent PolyJetTM showed that both luciferase and enhanced green fluorescent protein could be effectively expressed in RCFBF cells. The collagen-based hydrogel loaded with ZnO quantum dots can track the degradation process, inhibit the degradation process, have good biocompatibility with corneal cells, and achieve the purpose of gene delivery through the cells growing on its surface. It is a potential tissue engineering corneal material.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

【相似文献】