喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响

发布时间：2018-06-22 04:04

本文选题：喷砂酸蚀 + MCT-E　；参考：《口腔医学研究》2017年09期

【摘要】：目的:研究喷砂酸蚀(SLA)对钛及钛铌锆锡合金(Ti-24Nb-4Zr-7.9Sn,TNZS)表面形貌的影响,观察合金的形貌学特征,评价其生物相容性。方法:将试样分为钛机械打磨并抛光组(Ti组),钛铌锆锡机械打磨并抛光组(TNZS组),钛喷砂酸蚀组(Ti-SLA组)和钛铌锆锡喷砂酸蚀组(TNZS-SLA组),共4组。通过扫描电镜观察各组试样的表面形貌,3D激光共聚焦显微镜和接触角测量仪测量各组试样表面的粗糙度与亲水性。接种MC3T3-E1小鼠前成骨细胞于各组试样表面,检测细胞在试样表面的粘附、增殖与矿化的能力,评估其生物相容性。结果:SLA处理后在材料表面形成纳米级及微米级的凹坑,产生均匀分布的粗糙结构,经过处理后材料仍保持亲水性。细胞在TNZS组上短期粘附明显高于其它组(P0.05),TNZS-SLA组细胞增殖、分化能力均明显高于其它组(P0.05)。结论:喷砂酸蚀后材料表面相对于光滑材料表面能更有效的促进成骨细胞在其表面增殖、分化,经喷砂酸蚀的钛铌锆锡合金具有良好的细胞相容性。
[Abstract]:Aim: to investigate the effect of sand blast etching (SLA) on the surface morphology of titanium and Ti-24Nb-4Zr-7.9 SnNZS, to observe the morphologic characteristics of the alloys and to evaluate their biocompatibility. Methods: the samples were divided into four groups: titanium mechanical grinding and polishing group (Ti group), titanium niobium zirconium tin mechanical grinding and polishing group (TNZS group), titanium sandblasting acid etching group (Ti-SLA group) and titanium niobium zirconium tin sandblasting etching group (TNZS-SLA group). The surface morphology of each group was observed by scanning electron microscope (SEM). The surface roughness and hydrophilicity of each group were measured by 3D laser confocal microscope and contact angle measuring instrument. MC3T3-E1 mouse preosteoblasts were inoculated on the surface of each group. The ability of adhesion, proliferation and mineralization of the cells on the surface of the sample was detected, and the biocompatibility of the cells was evaluated. Results the nano-scale and micron size pits were formed on the surface of the material after the treatment of w / SLA, resulting in the uniform distribution of rough structure, and the hydrophilicity of the material was maintained after the treatment. The short-term adhesion of cells in TNZS group was significantly higher than that in other groups (P0.05), and the differentiation ability of cells in TNZS-SLA group was significantly higher than that in other groups (P0.05). Conclusion: compared with smooth surface, the surface of sand-blast acid etched material can promote the proliferation and differentiation of osteoblasts on its surface, and the titanium, niobium, zirconium and tin alloy etched by sandblasting has good cytocompatibility.
【作者单位】：哈尔滨医科大学口腔医学院牙体牙髓病科;
【基金】：黑龙江省卫生厅科研课题资助项目(编号:2011-051)
【分类号】：R783.1

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