SDF-1α复合Pluronic F-127的构建及其对BMSCs的体外生物效应研究

发布时间：2018-06-25 04:15

本文选题：基质细胞衍生因子-α + Pluronic　；参考：《安徽医科大学学报》2014年08期

【摘要】：目的构建装载基质细胞衍生因子-1α(SDF-1α)的Pluronic F-127新型复合材料,并研究其对骨髓间充质干细胞(BMSCs)的体外生物效应。方法构建装载不同浓度SDF-1α(200、50 ng/ml)的Pluronic F-127复合水凝胶,采用Transwell小室进行复合水凝胶对BMSCs的体外趋化实验。将BMSCs与复合材料均匀混合,加成骨细胞诱导液进行培养,并对其行碱性磷酸酶(AKP)活性测定及茜素红染色,由结果可观察到BMSCs的成骨诱导分化情况,并用MTT法检测复合培养后细胞的活性状况。结果趋化实验结果显示,含SDF-1α浓度为200 ng/ml的复合水凝胶对BMSCs的募集效果显著(P0.05)。MTT法证实了Pluronic F-127对细胞增殖不产生影响(P0.05)。BMSCs-复合材料、单纯BMSCs在成骨诱导培养条件下,两组细胞的AKP表达量均明显增高,并且都高于非成骨诱导组(P0.05),同时诱导组镜下可见明显的钙结节形成。结论成功构建装载SDF-1α的Pluronic F-127新型复合材料,其在体外对BMSCs有明显的趋化作用,在细胞材料复合培养的三维支架中,BMSCs能够存活并被成功诱导分化为成骨细胞。
[Abstract]:Objective to construct a novel Pluronic F-127 composite loaded with stromal cell derived factor-1 伪 (SDF-1 伪) and study its biological effects on bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods Pluronic F-127 composite hydrogels loaded with different concentrations of SDF-1 伪 (200,50 ng/ml) were constructed and chemotaxis of BMSCs in vitro were carried out by Transwell chamber. BMSCs were homogeneously mixed with composites and cultured with osteoblast inducer. The osteogenic differentiation of BMSCs was observed by alkaline phosphatase (AKP) activity and alizarin red staining. MTT assay was used to detect the activity of the cells after co-culture. Results the results of chemotaxis experiment showed that the recruitment effect of the composite hydrogel containing SDF-1 伪 was significant (P0.05). The results showed that Pluronic F-127 had no effect on cell proliferation (P0.05). BMSCs were cultured in osteogenic culture condition. The expression of AKP in both groups was significantly higher than that in non-osteogenic induction group (P0.05). At the same time, calcium nodule formation could be seen in the induction group under microscope. Conclusion Pluronic F-127 composite loaded with SDF-1 伪 has obvious chemotactic effect on BMSCs in vitro. BMSCs can survive and be induced to differentiate into osteoblasts in three dimensional scaffolds.
【作者单位】：安徽医科大学口腔医学院;安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;安徽医科大学附属口腔医院口腔颌面外科;
【基金】：国家自然科学基金(编号:81070864)
【分类号】：R318.08

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