低强度脉冲超声波对富血小板纤维蛋白释放生长因子的影响

发布时间：2018-06-30 04:52

本文选题：低强度脉冲超声波 + 富血小板纤维蛋白　；参考：《延边大学》2012年硕士论文

【摘要】：目的：探讨低强度脉冲超声波(LIPUS)对富血小板纤维蛋白(PRF)释放3种生长因子(碱性成纤维细胞生长因子(bFGF),转化生长因子p1(TGF-p1),表皮生长因子(EGF))的影响,为今后更有效的应用PRF提供实验依据和理论基础。方法：1.选择10名不吸烟的健康成人(22-27岁)男性志愿者,抽静脉血20ml加入无任何抗凝剂的采血管中,即刻以3000r/min离心10分钟,获得初级的PRF凝胶,再将其静置于干燥消毒的容器内10min,使其自然收缩并释放其内的血清,然后用LIPUS辐照PRF所释放的渗出液。2.根据辐照时间分为3组：分别为辐照LIPUS15分钟组(L-15min组)、辐照LIPUS30分钟组(L-30mmin组)和空白对照组(Control组)。3.实验组和对照组采用酶联免疫吸附法(ELISA)测定PRF渗出液中的3种生长因子含量。所得数据用SPSS18.0软件进行统计学分析。结果：低强度脉冲超声波作用于富血小板纤维蛋白后3种生长因子浓度变化：bFGF的L-15min组(318.60pg/ml)大于L-30min组(293.00pg/ml)和Control组(278.20pg/ml),3组之间差异均有显著性意义(P0.05); TGF-β1的L-15min组(7259pg/ml)大于L-30min组(6035pg/ml)和Control组(5503pg/ml),3组之间差异均有显著性意义(P0.05); EGF的Control组、L-15min组和L-30min组平均浓度分别为177.87pg/ml、181.80pg/ml和177.91pg/ml,3组之间浓度变化均无明显差异(P0.05)。结论：低强度脉冲超声波可能使富血小板纤维蛋白所释放的生长因子的浓度增加。在临床治疗中,富血小板纤维蛋白联合低强度脉冲超声波,应用于牙周组织再生修复,能更有效的利用富血小板纤维蛋白。
[Abstract]:Objective: to investigate the effects of low intensity pulsed ultrasound (LIPUS) on the release of three growth factors (basic fibroblast growth factor (bFGF), transforming growth factor p1 (TGF-p1), epidermal growth factor (EGF) by platelet-rich fibrin (PRF). It provides experimental and theoretical basis for more effective application of PRF in the future. Method 1: 1. Ten healthy non-smoking male volunteers (22-27 years old) were enrolled in this study. 20ml was extracted from venous blood and added to the blood vessels without any anticoagulant. The primary gel was obtained by centrifugation with 3000r/min for 10 minutes. Then it was statically placed in a dry sterilized container for 10 mins to naturally contract and release the serum in it. Then LIPUS was used to irradiate the exudate of PRF. 2. According to the irradiation time, the rats were divided into three groups: irradiation LIPUS 15-minute group (L-15min group), irradiated LIPUS30-minute group (L-30mmin group) and blank control group (Control group) .3. Enzyme linked immunosorbent assay (Elisa) was used to determine the content of three growth factors in PRF exudate. The data were analyzed by SPSS 18.0 software. Results: there were significant differences between L-15min group (318.60pg/ml) and control group (278.20pg/ml) and L-15min group (7259pg/ml) of TGF- 尾 1 compared with L-30min group (P0.05) and L-15min group (7259pg/ml) with TGF- 尾 1 as compared with L-30min group (P 0.05). There were significant differences between 6035pg/ml and 5503pg/ml groups (P0.05), but there was no significant difference between the control group (177.87pgml / ml) and the control group (177.91pgml / ml) (P0.05). Conclusion: low intensity pulsed ultrasound may increase the concentration of growth factor released by platelet rich fibrin. In clinical treatment, platelet-rich fibrin combined with low-intensity pulsed ultrasound can be used in periodontal tissue regeneration and repair, and it can be used more effectively to make use of platelet-rich fibrin.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R310

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