缺损颅骨组织工程支架生物力学分析及其体外整合实验研究

发布时间：2018-06-30 05:33

本文选题：颅骨组织工程 + 支架　；参考：《天津大学》2012年硕士论文

【摘要】：缺损颅骨修复在骨组织工程技术中占有重要地位，颅骨缺损部位修复所需的组织工程支架目前仍处于研究阶段。支架的生物力学性能和生物学性能是支架能否用于临床的重要内容。本文在已工作基础上对这两个方面进行深入研究，主要内容如下。首先，基于已有数据，构建了不同孔隙率支架，对支架的生物力学性能进行有限元与实验分析，应用快速原型、凝胶注模技术制作具有生物活性的三维颅骨组织工程支架。其次，通过引入高温玻璃粘接剂，并结合正交试验优化凝胶注模工艺参数，提高支架的力学性能。在凝胶注模实验过程中，，分析了高温玻璃粘结剂的添加，及添加顺序对支架收缩性能产生影响。最后，使用扫描电子显微镜，能谱分析，X射线衍射分析以及细胞体外整合实验对支架的生物学性能进行检测，验证所设计制造的缺损颅骨组织工程支架的生物相容性。测试结果证明，孔隙率为36%的三维支架的力学性能最优，-TCP/BG支架的力学强度及其材料成分满足进行细胞的培养的要求，为颅骨组织工程支架的应用及发展奠定了理论基础。
[Abstract]:Skull defect repair plays an important role in bone tissue engineering, and the tissue engineering scaffold for skull defect repair is still in the research stage. Biomechanical properties and biological properties of scaffolds are important for clinical application. Based on the work done, this paper makes a deep study of these two aspects, the main contents are as follows. Firstly, based on the available data, the scaffolds with different porosity were constructed. The biomechanical properties of scaffolds were analyzed by finite element method and experimental analysis. The scaffolds with biological activity were fabricated by rapid prototyping and gel injection. Secondly, the mechanical properties of the scaffold were improved by introducing high temperature glass binder and combining with orthogonal test to optimize the parameters of gel injection molding process. The effects of the addition of high temperature glass binder and the addition order on the shrinkage of the scaffold were analyzed. Finally, the biocompatibility of the scaffold was tested by scanning electron microscope (SEM), energy dispersive X-ray diffraction (EDS) and cell integration in vitro to verify the biocompatibility of the scaffold. The test results show that the mechanical properties of the three-dimensional scaffolds with porosity of 36% are optimal and the mechanical strength and material composition of the scaffolds meet the requirements of cell culture, which lays a theoretical foundation for the application and development of scaffolds for skull tissue engineering.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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