仿生多层骨软骨支架材料的制备

发布时间：2018-07-02 09:13

本文选题：胶原 + 骨软骨支架　；参考：《中国地质大学(北京)》2012年硕士论文

【摘要】：关节软骨在人体中所处位置特殊,频繁受到高强度外力的作用,导致软骨发生损伤的几率是很高的。但是,软骨自身修复能力有限,一旦发生损伤病变,便很难恢复,甚至还会导致软骨退化。本课题利用组织工程学的基本理论,制备一种仿生多层骨软骨支架材料。将支架材料植入受损的关节软骨处,从而起到修复软骨损伤的作用。本课题选择生物相容性良好、可降解性能优异并在该领域已被广泛使用的鼠尾胶原和壳聚糖作为支架制备的原料,配置胶原/壳聚糖混合溶液,通过冷冻干燥法使混合溶液干燥成型,作为支架材料的软骨层。然后与本课题组成熟的聚乳酸(PLA)/骨粉骨材料结合制备得到仿生多层骨软骨支架材料：在扫描电镜下分别观察支架材料的软骨层和骨层的形貌：结果表明,软骨层具有既有垂直孔,又有圆孔的多层结构,骨层孔洞表面粗糙。这些形貌特点决定了具备这种形貌结构的支架材料有利于细胞的附着生长。提取小鼠的软骨细胞,将软骨细胞接种到多层软骨支架材料上。观察发现,细胞攀爬、附着在支架的各个位置,支架材料的多层结构对细胞的生长状态起引导作用,这说明通过实验方法制备的支架细胞相容性良好,利于软骨细胞的取向生长。对制备的仿生多层骨软骨支架材料进行热原性、细胞毒性、体外毒性测试、以及骨植入实验等生物相容性研究,证明支架材料具有良好的生物相容性,可以对软骨损伤进行修复。该仿生多层骨软骨支架材料具有广阔的应用前景。
[Abstract]:Articular cartilage is in a special position in the human body and is frequently affected by high intensity external force, which leads to a high probability of cartilage damage. However, the repair ability of cartilage itself is limited, once damage occurs, it is difficult to recover, and even lead to cartilage degeneration. Based on the basic theory of tissue engineering, a biomimetic multilayer bone cartilage scaffold was prepared. The scaffold material is implanted into the damaged articular cartilage to repair the cartilage injury. In this paper, we choose rat tail collagen and chitosan, which have good biocompatibility, excellent biodegradability and have been widely used in this field, as raw materials for scaffold preparation, and configure collagen / chitosan mixed solution. The mixed solution was dried by freeze-drying method and used as the cartilage layer of the scaffold. Then the biomimetic multilayer bone cartilage scaffold was prepared by combining with the mature PLA / bone powder bone material. The morphology of the cartilage layer and bone layer of the scaffold was observed under scanning electron microscope. The cartilage layer has a multilayer structure of both vertical and round holes, and the surface of the holes in the bone layer is rough. These morphologies determine that scaffolds with this structure are conducive to cell adhesion and growth. The chondrocytes of mice were extracted, and the chondrocytes were inoculated into multilayer cartilage scaffolds. It was found that cell climbing and attachment to every position of the scaffold, the multilayer structure of scaffold material played a guiding role in the cell growth state, which indicated that the scaffold prepared by the experimental method had good cell compatibility. It is beneficial to the growth of chondrocytes. The biocompatibility of the biomimetic multilayer bone cartilage scaffold was studied, such as pyrogenicity, cytotoxicity, in vitro toxicity test and bone implantation experiment, which proved that the scaffold had good biocompatibility. Cartilage damage can be repaired. The biomimetic multilayer bone cartilage scaffold has a broad application prospect.
【学位授予单位】：中国地质大学(北京)
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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