硅基纳米材料的生物传感应用和硅纳米线的毒性评价

发布时间：2018-07-02 13:41

本文选题：硅基纳米材料 + 表面增强拉曼散射　；参考：《苏州大学》2012年硕士论文

【摘要】：硅纳米材料是一种重要的半导体纳米材料，它具有优异的电学和机械性能、良好的生物相容性、较大的比表面积、表面易修饰性，以及与传统的硅科技的兼容性。因此，人们对发展功能化的硅纳米材料有着浓厚的兴趣，并已将其广泛应用于电子学和生物学领域。本文主要发展了基于硅基纳米材料的表面增强拉曼散射（surface-enhanced Raman scattering, SERS）基质，并用于DNA的高灵敏、特异性多元检测；还研究了硅纳米线在细胞层面的生物学效应，主要研究内容如下： 1、我们制备了一种基于硅基纳米材料的表面增强拉曼散射基质，即在硅片上沉积了一层银纳米粒子（silver nanoparticles-decorated silicon wafers,AgNPs@Si），并将其用于进行高灵敏、特异性的DNA多元检测。所制备AgNPs@Si具有极好的SERS信号重现性和较高的增强因子。我们的实验结果进一步的证明了这种硅基SERS基底能够有效地进行DNA的高灵敏、特异性多元检测。该研究对于发展具有良好重现性的SERS基质，发展基于SERS的生物分子检测方法具有积极的意义。 2、我们采用3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)细胞毒性测试、荧光成像和流式细胞术等经典生物学方法，，系统研究了三种不同表面性质的硅纳米线对肿瘤细胞系和正常细胞株在不同条件下（如硅纳米线浓度、共孵育时间等）的细胞毒性。研究结果发现：细胞毒性对硅纳米线浓度和共孵育时间具有明显依赖性：随着硅纳米线浓度的增加和共孵育时间的延长，细胞活性呈明显下降趋势；在相同条件下，肿瘤细胞株系较正常细胞株系具有更高的细胞活性；硅纳米线表面性质对细胞活性具有明显的影响。在此基础上,我们给出了硅纳米线用于细胞层面生物应用的安全阈值。
[Abstract]:Silicon nanomaterials are important semiconductor nanomaterials. They have excellent electrical and mechanical properties, good biocompatibility, large specific surface area, easy surface modification, and compatibility with traditional silicon technology. Therefore, there is a strong interest in the development of functionalized silicon nanomaterials, which have been widely used in electronics and biology. In this paper, the surface enhanced Raman scattering (SERS) matrix based on silicon-based nanomaterials has been developed and used for high sensitivity and specific multivariate detection of surface-enhanced, and the biological effects of silicon nanowires at the cellular level have also been studied. The main research contents are as follows: 1. We have prepared a surface-enhanced Raman scattering substrate based on silicon-based nanomaterials, that is, we deposited a silver nanoparticles-decorated silicon wafers AgNPs @ Si on silicon wafers and used it for high sensitivity. Specific DNA multivariate detection. The prepared AgNPs @ Si has excellent SERS signal reproducibility and high enhancement factor. Our experimental results further demonstrate that the Si-based SERS substrate can be used to detect DNA with high sensitivity and specificity. This study has positive significance for the development of SERS substrates with good reproducibility and the development of SERS-based biomolecules detection methods. 2. We used classical biological methods, such as 3- (4t5) -dimethylthiahiazo (-z-y1) -3-di-phenytetrazoliumromide (MTT) cytotoxicity test, fluorescence imaging and flow cytometry, etc. The cytotoxicity of three kinds of silicon nanowires with different surface properties to tumor cell lines and normal cell lines under different conditions such as the concentration of silicon nanowires and co-incubation time were studied. The results showed that the cytotoxicity was significantly dependent on the concentration and co-incubation time of silicon nanowires: with the increase of the concentration of silicon nanowires and the prolongation of co-incubation time, the cell activity decreased obviously. The cell activity of tumor cell line was higher than that of normal cell line, and the surface property of silicon nanowire had obvious influence on cell activity. On this basis, we give the safety threshold of silicon nanowires for biological applications at cell level.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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