同种异体和异种来源骨髓间充质干细胞诱导成软骨修复喉软骨缺损

发布时间：2018-07-02 21:53

  本文选题：喉软骨 + 骨髓　； 参考：《中国组织工程研究》2016年07期

【摘要】：背景:由于软骨细胞缺乏再生能力,选择合适的种子细胞是修复软骨缺损需首要解决的问题。目的:探讨同种异体和异种来源骨髓间充质干细胞成软骨诱导后修复喉软骨缺损的效果。方法:取第3代人骨髓间充质干细胞和兔骨髓间充质干细胞加入软骨定向诱导液(含转化生长因子β1和骨形态发生蛋白)进行成软骨诱导,并滴加于聚乳酸-羟基乙酸共聚物支架上。取30只新西兰大白兔随机分为3组:空白对照组、人骨髓间充质干细胞修复组和兔骨髓间充质干细胞修复组,制备喉软骨缺损动物模型,分别植入生理盐水浸湿的聚乳酸-羟基乙酸共聚物支架,人骨髓间充质干细胞复合聚乳酸-羟基乙酸共聚物支架,兔骨髓间充质干细胞复合聚乳酸-羟基乙酸共聚物支架。术后4周和8周,免疫组化法检测喉部组织Ⅱ型胶原的表达。结果与结论:各组动物均呼吸通畅、正常,未出现喘鸣等,进食和活动情况良好,未出现化脓或者感染现象。术后4周和8周,人骨髓间充质干细胞修复组和兔骨髓间充质干细胞修复组的Ⅱ型胶原阳性率均显著高于空白对照组(P0.05)。人骨髓间充质干细胞修复组和兔骨髓间充质干细胞修复组间比较,差异无显著性意义(P0.05)。结果表明同种异体和异种来源的骨髓间充质干细胞成软骨诱导后进行兔喉软骨缺损修复均可以获得良好的效果,二者修复效果无显著差异。
[Abstract]:Background: due to the lack of regeneration ability of chondrocytes, the choice of suitable seed cells is the most important problem in repairing cartilage defects. Aim: to investigate the effect of cartilage formation induced by allogeneic and xenogeneic bone marrow mesenchymal stem cells (MSCs) in repairing laryngeal cartilage defects. Methods: the third generation of human bone marrow mesenchymal stem cells and rabbit bone marrow mesenchymal stem cells were added into the chondrocyte directed inducer (TGF- 尾 1 and bone morphogenetic protein) to induce cartilage formation. It was added to the poly (lactic acid-glycolic acid) copolymer scaffold. Thirty New Zealand rabbits were randomly divided into three groups: blank control group, human bone marrow mesenchymal stem cell repair group and rabbit bone marrow mesenchymal stem cell repair group. The scaffolds of poly (lactic acid-glycolic acid) copolymers, human bone marrow mesenchymal stem cells (BMSCs) and poly (lactic acid-glycolic acid) copolymers, and rabbit bone marrow mesenchymal stem cells (BMSCs) were implanted respectively. The expression of type 鈪，

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