体外评价新型根尖倒充填材料iRoot的生物学性能

发布时间：2018-07-05 19:36

本文选题：根管充填材料 + 牙瓷料　；参考：《北京大学学报(医学版)》2016年02期

【摘要】：目的:体外评价新型根尖倒充填材料iRoot BP plus和iRoot FS的生物学性能。方法:(1)将牙根预备成长3 mm、根管直径1 mm的试样,分别于根管内填充iRoot BP plus、iRoot FS和三氧化矿物凝聚体(mineral trioxide aggregate,MTA)。将制备好的试样置于模拟体液(simulated body fluid,SBF)中,用扫描电子显微镜观察暴露的材料表面矿物沉积情况,并通过X射线能谱对其表面形成的晶体进行元素分析,测定SBF的p H值随时间的变化。(2)将iRoot BP plus、iRoot FS和MTA制备成直径8 mm、高度2 mm的圆柱,制备DMEM浸提液,通过MTT实验观察材料对MG63细胞增殖活性的影响,并通过实时荧光定量PCR和碱性磷酸酶(alkaline phosphatase,ALP)染色分析材料对ALP基因及蛋白表达的影响。结果:(1)在SBF中浸泡24 h时,iRoot BP plus、iRoot FS和MTA表面均已有矿物沉积,14 d后有大量矿物沉积,钙∶磷比值分别为1.43、1.39和1.51;(2)iRoot BP plus、iRoot FS和MTA均可使SBF的p H升高,3周时分别为8.09±0.07、7.91±0.06和8.11±0.06;(3)以体积比为1∶5和1∶10稀释的iRoot BP plus、iRoot FS和MTA浸提液对MG63细胞的增殖无明显促进或抑制作用;(4)iRoot BP plus、iRoot FS和MTA组对MG63细胞ALP基因的表达均具有促进作用,ALP染色各组未见明显差异。结论:iRoot在模拟体液中可诱导矿物质沉积,促进MG63细胞的分化和矿化,具有良好的生物活性和促成骨作用。
[Abstract]:Objective: to evaluate the biological properties of iRoot BP plus and iRoot FS in vitro. Methods: (1) the specimens with 3 mm root preparation and 1 mm diameter root canal were filled with iRoot BP plus iRoot FS and (mineral trioxide aggregate MTA, respectively. The prepared samples were placed in simulated body fluid (simulated body fluidge). The surface mineral deposits of exposed materials were observed by scanning electron microscope (SEM), and the elements of crystals formed on the surface were analyzed by X-ray spectroscopy. The pH value of SBF was measured with time. (2) the cells were prepared into 8mm diameter and 2mm high cylinder by iRoot BP plusiRoot FS and MTA. The DMEM extract was prepared. The effect of the material on the proliferation of MG63 cells was observed by MTT assay. The effect of alkaline phosphatase on the expression of ALP gene and protein was analyzed by real-time fluorescence quantitative PCR and alkaline phosphatase (alkaline) staining. Results: (1) after immersion in SBF for 24 h, a large number of minerals were deposited on the surfaces of iRoot BP plusiRoot FS and MTA after 14 days. The ratio of calcium to phosphorus was 1.43 卤1.39 and 1.51 respectively; (2) the pH of SBF was 8.09 卤0.07 卤7.91 卤0.06 and 8.11 卤0.06 at 3 weeks, respectively; (2) the ratio of iRoot BP plusiRoot FS and MTA diluted at 1:5 and 1:10 did not significantly promote the proliferation of MG63 cells. (4) both iRoot BP plusiRoot FS and MTA groups could promote the expression of ALP gene in MG63 cells. ConclusionThe specific root can induce mineral deposition and promote differentiation and mineralization of MG63 cells in simulated body fluid. It has good biological activity and bone promoting effect.
【作者单位】：北京大学口腔医学院·口腔医院牙体牙髓科;
【基金】：国家自然科学基金项目(51372005)资助~~
【分类号】：R783.1

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