功能性PLGA纳米药物颗粒胶原蛋白膜在骨再生中的实验研究

发布时间：2018-07-07 22:04

本文选题：乳酸乙醇酸共聚物 + 地塞米松　；参考：《延边大学》2012年硕士论文

【摘要】：目的：这个研究的目的是开发一个功能性胶原蛋白膜,将涂有地塞米松作为生物活性药物的乳酸乙醇酸共聚物(poly-lactic-co-glycolic acid, PLGA)纳米颗粒的生物膜植入骨缺损区,研究引导骨组织再生(Guided Bone Regeneration, GBR)的影响。材料与方法：在这项研究中,含有地塞米松纳米药物颗粒,以电荷相互作用力固定于胶原蛋白膜表面。地塞米松作为生物活性药物的PLGA纳米颗粒,用water-in-oil乳液相态法制备。制作而成的纳米药物颗粒先用带有强的正电荷的聚乙二醇(poly-ethyleneimmine, PEI)均匀涂布处理后以电荷相互作用力固定于带有负电荷的生物膜表面。做4周的药物释放实验中最初释放率很高,之后呈一个持续性释放。此药物释放系统在大白鼠颅骨制备了直径为8mm的骨缺损区,并进行了临床前期的研究。植入PLGA纳米药物颗粒的可吸收性胶原蛋白膜的骨缺损区的实验组可见,新生骨的增多及骨密质度的增高明显高于空白组和只植入骨移植材料(Bio-Oss(?))植入骨缺损区后,植入未作任何处理的(Bio-Gide(?))胶原蛋白膜。结果：新型的生物活性分子释放平台在体内更有效地诱导新骨生成,利用植入式生物膜可以对于肝细胞疗法或提高在体内的性能。结论：新开发的PLGA纳米药物固定胶原膜系统,是一种很有前途的适用于骨再生的材料。
[Abstract]:Objective: the aim of this study was to develop a functional collagen membrane in which poly-lactic-co-glycolic glycolate nanoparticles coated with dexamethasone as a bioactive drug were implanted into the bone defect. To study the effect of guided bone regeneration (GBR). Materials and methods: in this study, dexamethasone nanoparticles were immobilized on the surface of collagen membrane by charge interaction. Dexamethasone as a bioactive drug PLGA nanoparticles were prepared by water-in-oil emulsion phase method. The prepared nanoparticles were uniformly coated with poly-ethyleneimmine (PEI) with strong positive charge and then fixed on the surface of biofilm with negative charge by charge interaction force. After 4 weeks of drug release, the initial release rate was high, followed by a sustained release. The drug release system was used to prepare the bone defect area with diameter of 8mm in the cranium of the rat, and a preliminary clinical study was carried out. The experimental group with absorbable collagen membrane implanted PLGA nanoparticles showed that the increase of new bone and the increase of bone density were significantly higher than those of the blank group and only implanted bone graft material (Bio-Oss (?). After implantation of bone defect area, implanted without any treatment (Bio-Gide (?) Collagen membrane. Results: the new bioactive molecular release platform can induce the formation of new bone more effectively in vivo. The bioactive biofilm can be used to treat hepatocytes or improve its performance in vivo. Conclusion: the newly developed PLGA nanopharmaceutical system for collagen membrane fixation is a promising material for bone regeneration.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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