组织工程骨膜及脱蛋白骨辅助支架修复兔桡骨大段骨缺损

发布时间：2018-07-07 21:36

本文选题：骨组织工程 + 组织工程骨膜　；参考：《中国修复重建外科杂志》2014年04期

【摘要】：目的评价组织工程骨膜体内成骨修复兔大段骨缺损及同种异体脱蛋白骨(deproteinized bone,DPB)作为组织工程骨膜辅助支架材料的效果。方法分离培养1月龄新西兰大白兔BMSCs,以成骨诱导培养液诱导成骨分化后,与猪小肠黏膜下层(small intestinal submucosa,SIS)复合构建组织工程骨膜,扫描电镜观察细胞与SIS复合情况。取新鲜同种异体骨段细密打孔后进行脱蛋白处理,制成DPB支架材料。将组织工程骨膜呈"外套状"包被DPB,并用可吸收缝线捆扎制备组织工程骨膜/DPB复合体。取4月龄清洁级新西兰大白兔48只,随机分成A、B、C、D 4组(n=12),切除左侧桡骨干3.5 cm长骨段制作大段骨缺损动物模型。A、B、C组分别于骨缺损处植入组织工程骨膜、DPB和组织工程骨膜/DPB复合体,D组骨缺损旷置。术后4、8、12周各组随机取4只动物行X线片观察及评分;术后8周各组随机处死4只动物,取骨缺损区标本行HE及Masson染色观察。结果扫描电镜观察构建的组织工程骨膜示SIS上黏附大量细胞。X线片检查示术后各时间点A、C组新生骨组织量明显多于B、D组。术后各时间点A、C组X线片评分显著高于B、D组,A组高于C组,B组高于D组,差异均有统计学意义(P0.05)。组织学观察示A组骨缺损处为大量新生骨组织,新生骨组织中可见不规则髓腔样结构及丰富的血管腔;B组骨缺损处见植入的DPB,DPB降解不明显;C组骨缺损处见较多新生骨组织,新生骨组织中可见呈岛状分布的DPB,DPB降解明显;D组骨缺损处仅见少量胶原纤维。结论以SIS和BMSCs构建的组织工程骨膜可修复兔桡骨大段骨缺损;DPB不能对组织工程骨膜成骨起到理想的支架作用,组织工程骨膜辅助支架材料有待进一步探索。
[Abstract]:Objective to evaluate the effect of in vivo osteogenesis of tissue engineered periosteum for repairing large segment bone defect and deproteinized bone dpb as an auxiliary scaffold for tissue engineering periosteum. Methods New Zealand rabbits (BMSCs) were isolated and cultured for 1 month. After osteogenic differentiation was induced by osteogenic medium, tissue engineered periosteum was constructed with porcine small intestinal submucosa (small intestinal submucosa. The composite of cells and SIS was observed by scanning electron microscope (SEM). The fresh allogeneic bone segment was drilled and then deproteinized to make dpb scaffold. Tissue engineering periosteum / dpb complex was prepared by wrapping the tissue engineered periosteum with "overcoat" and using absorbable suture to bind the tissue engineered periosteum to form a tissue engineered periosteum / dpb complex. 48 New Zealand white rabbits of 4 months old clean grade were selected. The animal model of large segment bone defect was made by resection of 3.5 cm long bone segment of left radius. Group C was implanted with tissue engineered periosteum dpb and tissue engineered periosteum / dpb complex in group D respectively. At 12 weeks after operation, 4 animals in each group were randomly selected for X-ray observation and scored, 4 animals were killed at random at 8 weeks after operation, and the specimens of bone defect area were observed by HE and Masson staining. Results the tissue engineered periosteum showed a large number of cells attached to SIS by scanning electron microscope. X-ray examination showed that the amount of new bone tissue in group A was significantly higher than that in group B D at each time point after operation. The X ray score of group A was significantly higher than that of group A (P 0.05), and the score of group B was higher than that of group B (P 0.05). Histopathological observation showed that a large number of new bone tissues were found in the bone defects in group A, irregular medullary cavities and abundant vascular cavities were found in the bone defects in group A, and there was no obvious degradation of DPB-dpb in group B. in group C, there were more new bone tissues in bone defects. In group D, only a small amount of collagen fibers were found in the bone defect of group D. Conclusion the tissue engineered periosteum constructed with SIS and BMSCs can repair the defect of rabbit radial bone and dpb can not play an ideal scaffold role in tissue engineering periosteum osteogenesis, and the tissue engineering periosteum auxiliary scaffold material needs further exploration.
【作者单位】：兰州大学第二医院骨科甘肃省骨与关节疾病重点实验室;兰州大学第二医院妇科;
【基金】：国家自然科学基金资助项目(30973064)~~
【分类号】：R318.08

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