基于咪唑—锌离子作用的形状记忆水凝胶的构建及其作为管状细胞支架的应用探索
发布时间：2018-07-31 08:07
【摘要】：本文利用一步法以聚乙二醇二丙烯酸酯(PEGDA)为交联剂，1-乙烯基咪唑和丙烯腈为单体共聚合成一种偶极-偶极增强水凝胶。该水凝胶的力学强度在微量锌离子溶液中显著提高，进一步研究发现凝胶和少量金属离子的络合作用能够固定住临时形状，去除离子可使凝胶恢复原始形状，从而实现形状记忆功能，而且这种记忆特性是可重复的。此外，此水凝胶有良好的细胞相容性，细胞能在该水凝胶上很好的生长。将长有细胞的水凝胶平片卷成管状，然后放置在5mmol/L锌离子培养基中固定形状，放入细胞培养基中培养一段打开管状凝胶支架发现，细胞仍能很好的粘附在凝胶上并保持正常形态。这种包含在凝胶中的咪唑环配体和微量锌离子的络合-解络合策略提供了一种实现形状记忆凝胶的简单且普遍的方法，并且由于其实现过程对细胞没有大的伤害，，很有希望作为一种可植入组织工程支架应用于生物领域。
[Abstract]:A dipole-dipole reinforced hydrogel was prepared by one-step copolymerization of poly (ethylene glycol diacrylate) (PEGDA) as crosslinking agent and acrylonitrile as monomer. The mechanical strength of the hydrogel was significantly increased in trace zinc ion solution. It was found that the complexation of the gel with a small amount of metal ions could fix the temporary shape, and the removal of ions could restore the original shape of the gel. Thus the shape memory function is realized, and the memory property is repeatable. In addition, the hydrogel has good cell compatibility and cells can grow well on the hydrogel. The hydrogel flattens with long cells were rolled into tubules, then placed in 5mmol/L zinc ion medium to fix the shape, and cultured in the cell culture medium for a section of open tubular gel scaffold. Cells still adhere well to the gel and maintain normal morphology. This complexation-desorption strategy of imidazole ring ligands and trace zinc ions in the gel provides a simple and universal method for realizing shape memory gel, and because of its implementation process, it does not cause much damage to cells. It is promising to be used as an implantable scaffold for tissue engineering in biological field.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08
本文编号：2154956
[Abstract]:A dipole-dipole reinforced hydrogel was prepared by one-step copolymerization of poly (ethylene glycol diacrylate) (PEGDA) as crosslinking agent and acrylonitrile as monomer. The mechanical strength of the hydrogel was significantly increased in trace zinc ion solution. It was found that the complexation of the gel with a small amount of metal ions could fix the temporary shape, and the removal of ions could restore the original shape of the gel. Thus the shape memory function is realized, and the memory property is repeatable. In addition, the hydrogel has good cell compatibility and cells can grow well on the hydrogel. The hydrogel flattens with long cells were rolled into tubules, then placed in 5mmol/L zinc ion medium to fix the shape, and cultured in the cell culture medium for a section of open tubular gel scaffold. Cells still adhere well to the gel and maintain normal morphology. This complexation-desorption strategy of imidazole ring ligands and trace zinc ions in the gel provides a simple and universal method for realizing shape memory gel, and because of its implementation process, it does not cause much damage to cells. It is promising to be used as an implantable scaffold for tissue engineering in biological field.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08
【参考文献】
相关期刊论文 前3条
1 李贤真,李彦锋,朱晓夏,李柏年,刘刚;高分子水凝胶材料研究进展[J];功能材料;2003年04期
2 柳明珠,吴靖嘉,宫伟;甲基丙烯酸甲酯与马铃薯淀粉的接枝共聚合[J];合成橡胶工业;1993年04期
3 李志军;王振武;宋宏立;;智能水凝胶的研究进展[J];化学工业与工程技术;2006年01期
本文编号：2154956
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