用于引导牙周组织再生壳聚糖基隔离膜的制备及其表征
发布时间：2018-08-01 17:02
【摘要】：牙周炎是一种导致牙周组织逐渐损坏近而引起成人牙齿缺失的炎症疾病。一种治疗牙周病的理想结果就是在采用牙周再生技术进行牙周病治疗引起感染之前使牙周组织长回原位,以恢复其原始结构和功能。骨移植和引导组织再生技术是近年来治疗牙周组织缺损的重要手段。本论文采用聚乙烯醇(PVA)对壳聚糖(CS)膜进行改性以提高其实际应用价值；将冰硼散(BPS)载入到CS膜中避免引导牙周组织再生过程中引起细菌感染；将骨移植法和引导组织再生技术结合起来促进CS膜的生物活性。 (1)溶液浇注法制备CS/PVA复合膜 采用溶液浇铸法制备了CS/PVA复合膜。综合研究了复合膜的化学性质、物理性能、热稳定性能、表面润湿性能、力学性能、降解性能以及生物相容性。研究发现CS与PVA通过形成分子间和分子内氢键具有强的相互作用。当CS含量占主要成分时,CS与PVA的相容性良好；当PVA含量近一步增加时出现一定程度的相分离。添加PVA可以大大提高CS膜的韧性和改善表面润湿性能,调控CS膜的降解性能以及降解过程中表面形貌。CS/PVA复合膜的生物相容性良好,在引导组织再生领域具有潜在的应用价值。 (2)凝胶法制备CS/BPS复合膜 采用凝胶法制备出CS/BPS复合膜。该方法避免了BPS在CS膜后处理过程中受到酸碱的影响,保持了药物活性。添加BPS使得CS分子内和分子间氢键密度降低,增加了自由氨基和羟基含量。当CS与BPS质量比较高时,CS与BPS混合性良好,无明显相分离；当其质量比较低时,混合性变差,出现相分离。CS/BPS复合膜表面润湿性能良好,力学性能能够满足引导牙周组织再生应用。CS/BPS复合膜能够促进细胞在其表面的铺展、生长和增殖。 (3)电化学法仿生矿化CS凝胶制备有机／无机杂化材料 以CS凝胶为模板采用电化学法制备出仅一边具有生物活性矿物的不对称结构的壳聚糖／羟基磷灰石／碳酸钙杂化材料。CS氨基与钙离子络合作用较强,pH值对矿物形成具有重大影响。阳极一侧无矿物产生,阴极一侧CS凝胶表面产生直径5-6μm矿物粒子,并且单一粒子均有几纳米大小的颗粒组成。与将材料浸入到模拟体液进行生物矿化的方法相比,该方法大大降低了矿化时间。随着矿化时间增加,Ca/P比逐渐降低,但高于1.65(羟基磷灰石的Ca/P比)。高Ca/P比能够确保有机/无机杂化材料具有较高含量的Ca和足够多的P来维持较高水平的骨矿物密度。
[Abstract]:Periodontitis is an inflammatory disease that causes periodontal tissue damage and tooth loss in adults. An ideal outcome for the treatment of periodontal disease is to restore the periodontal tissue to its original structure and function before periodontal regeneration is used to treat the infection. Bone transplantation and guided tissue regeneration are important methods for the treatment of periodontal tissue defects in recent years. In this paper, polyvinyl alcohol (PVA) was used to modify chitosan (CS) membrane to improve its practical application value, and the (BPS) was loaded into CS membrane to avoid bacterial infection during periodontal tissue regeneration. Bone graft and guided tissue regeneration were combined to promote the biological activity of CS membrane. (1) CS/PVA composite membrane was prepared by solution casting method and CS/PVA composite membrane was prepared by solution casting method. The chemical properties, physical properties, thermal stability, surface wettability, mechanical properties, degradation properties and biocompatibility of the composite films were studied. It is found that CS and PVA interact strongly by forming intermolecular and intramolecular hydrogen bonds. The compatibility between CS and PVA was good when CS content was the main component, and the phase separation appeared when the content of PVA increased one step later. The addition of PVA can greatly improve the toughness and wettability of CS film, and regulate the degradation performance of CS film and the biocompatibility of CS- / PVA composite film during the degradation process. It has potential application value in guiding tissue regeneration. (2) CS/BPS composite membrane was prepared by gel method and CS/BPS composite membrane was prepared by gel method. This method avoids the influence of acid and base on BPS during CS membrane aftertreatment, and maintains the drug activity. The addition of BPS reduced the intramolecular and intermolecular hydrogen bonding densities of CS and increased the free amino and hydroxyl contents. When the mass ratio of CS to BPS is high, the mixture of CS and BPS is good, and when the mass is low, the mixing property becomes worse, and the surface wettability of CS- / BPS composite membrane is good. Mechanical properties can be used to guide periodontal tissue regeneration. CS- / BPS composite membrane can promote cell spreading on its surface. (3) preparation of organic / inorganic hybrid materials by electrochemical biomimetic mineralized CS gel using CS gel as template to prepare asymmetric junctions with only one side of biologically active minerals prepared by electrochemical method Chitosan / hydroxyapatite / calcium carbonate hybrid material. CS amino complex with calcium ions has a strong interaction. The pH value has a significant impact on mineral formation. No minerals were produced on the anode side, and the surface of CS gel on the cathode side produced 5-6 渭 m mineral particles in diameter, and the single particles were composed of several nanometer-sized particles. Compared with the method of imitating body fluid for biomineralization, this method greatly reduces the time of mineralization. The Ca / P ratio decreased gradually with the increase of mineralization time, but was higher than 1.65 (Ca/P ratio of hydroxyapatite). High Ca/P ratio can ensure that organic / inorganic hybrid materials have high Ca content and sufficient P to maintain high bone mineral density.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TB383.2;R783.1
本文编号：2158242
[Abstract]:Periodontitis is an inflammatory disease that causes periodontal tissue damage and tooth loss in adults. An ideal outcome for the treatment of periodontal disease is to restore the periodontal tissue to its original structure and function before periodontal regeneration is used to treat the infection. Bone transplantation and guided tissue regeneration are important methods for the treatment of periodontal tissue defects in recent years. In this paper, polyvinyl alcohol (PVA) was used to modify chitosan (CS) membrane to improve its practical application value, and the (BPS) was loaded into CS membrane to avoid bacterial infection during periodontal tissue regeneration. Bone graft and guided tissue regeneration were combined to promote the biological activity of CS membrane. (1) CS/PVA composite membrane was prepared by solution casting method and CS/PVA composite membrane was prepared by solution casting method. The chemical properties, physical properties, thermal stability, surface wettability, mechanical properties, degradation properties and biocompatibility of the composite films were studied. It is found that CS and PVA interact strongly by forming intermolecular and intramolecular hydrogen bonds. The compatibility between CS and PVA was good when CS content was the main component, and the phase separation appeared when the content of PVA increased one step later. The addition of PVA can greatly improve the toughness and wettability of CS film, and regulate the degradation performance of CS film and the biocompatibility of CS- / PVA composite film during the degradation process. It has potential application value in guiding tissue regeneration. (2) CS/BPS composite membrane was prepared by gel method and CS/BPS composite membrane was prepared by gel method. This method avoids the influence of acid and base on BPS during CS membrane aftertreatment, and maintains the drug activity. The addition of BPS reduced the intramolecular and intermolecular hydrogen bonding densities of CS and increased the free amino and hydroxyl contents. When the mass ratio of CS to BPS is high, the mixture of CS and BPS is good, and when the mass is low, the mixing property becomes worse, and the surface wettability of CS- / BPS composite membrane is good. Mechanical properties can be used to guide periodontal tissue regeneration. CS- / BPS composite membrane can promote cell spreading on its surface. (3) preparation of organic / inorganic hybrid materials by electrochemical biomimetic mineralized CS gel using CS gel as template to prepare asymmetric junctions with only one side of biologically active minerals prepared by electrochemical method Chitosan / hydroxyapatite / calcium carbonate hybrid material. CS amino complex with calcium ions has a strong interaction. The pH value has a significant impact on mineral formation. No minerals were produced on the anode side, and the surface of CS gel on the cathode side produced 5-6 渭 m mineral particles in diameter, and the single particles were composed of several nanometer-sized particles. Compared with the method of imitating body fluid for biomineralization, this method greatly reduces the time of mineralization. The Ca / P ratio decreased gradually with the increase of mineralization time, but was higher than 1.65 (Ca/P ratio of hydroxyapatite). High Ca/P ratio can ensure that organic / inorganic hybrid materials have high Ca content and sufficient P to maintain high bone mineral density.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TB383.2;R783.1
【参考文献】
相关硕士学位论文 前1条
1 常秀梅;组织工程方法构建牙周组织的实验研究[D];第一军医大学;2005年
，本文编号：2158242
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2158242.html
