酶交联明胶水凝胶性能研究及仿生微流道制备
发布时间：2018-08-01 19:05
【摘要】：针对当前水凝胶构建实质性功能器官存在制备血管网络困难的问题,采用了转谷氨酰胺酶(mTG酶)交联明胶构建明胶水凝胶圆形仿生微流道的方法。研究了明胶水凝胶的力学性能、成形性能及生物性能与明胶浓度的关系,提出了明胶水凝胶构建微流道结构的工艺条件,评价了微流道构建工艺的成型精度、力学性能和内皮化功能。结果表明:随着水凝胶浓度的提高,水凝胶的力学性能成线性增加,微结构的成型性能也逐步改善,但是浓度过高生物性能反而降低,明胶质量浓度为0.1g/mL的水凝胶综合性能相对较好,可用于构建圆形仿生微流道;采用的微流道成型工艺保持了原有水凝胶的力学性能,但是存在一定的成型误差,细胞在微流道内分布均匀,实现了微流道的内皮化。通过研究不同浓度的酶交联明胶水凝胶,制备的仿生微流道有望用于实质性功能器官的仿生再建。
[Abstract]:In view of the difficulty of preparing vascular network in the construction of solid functional organs by hydrogels, the circular bionic microchannels of gelatin hydrogels were constructed by transglutaminase (mTG) cross-linking gelatin. The relationship between the mechanical properties, formability and biological properties of gelatin hydrogels and the concentration of gelatin was studied. The technological conditions for the construction of microchannel structures by gelatin hydrogels were put forward, and the forming accuracy of the microchannel construction process was evaluated. Mechanical properties and endothelial function. The results show that with the increase of hydrogel concentration, the mechanical properties of hydrogels increase linearly, and the molding properties of microstructures are improved gradually, but the biological properties of hydrogels with high concentration decrease. The hydrogels with gelatin concentration of 0.1g/mL have relatively good comprehensive properties, and can be used to construct circular bionic microchannels. The mechanical properties of the original hydrogels are maintained by the microchannel molding process, but there are some forming errors. The cells were evenly distributed in the microchannel and the endothelialization of the microchannel was realized. By studying gelatin hydrogels with different concentrations of enzyme crosslinking, the biomimetic microchannels are expected to be used in the bionic reconstruction of solid functional organs.
【作者单位】： 西安交通大学机械制造系统工程国家重点实验室;
【基金】：国家自然科学基金资助项目(51675416) 陕西省统筹项目(2015KTTSGY-04)
【分类号】：R318.08
本文编号：2158541
[Abstract]:In view of the difficulty of preparing vascular network in the construction of solid functional organs by hydrogels, the circular bionic microchannels of gelatin hydrogels were constructed by transglutaminase (mTG) cross-linking gelatin. The relationship between the mechanical properties, formability and biological properties of gelatin hydrogels and the concentration of gelatin was studied. The technological conditions for the construction of microchannel structures by gelatin hydrogels were put forward, and the forming accuracy of the microchannel construction process was evaluated. Mechanical properties and endothelial function. The results show that with the increase of hydrogel concentration, the mechanical properties of hydrogels increase linearly, and the molding properties of microstructures are improved gradually, but the biological properties of hydrogels with high concentration decrease. The hydrogels with gelatin concentration of 0.1g/mL have relatively good comprehensive properties, and can be used to construct circular bionic microchannels. The mechanical properties of the original hydrogels are maintained by the microchannel molding process, but there are some forming errors. The cells were evenly distributed in the microchannel and the endothelialization of the microchannel was realized. By studying gelatin hydrogels with different concentrations of enzyme crosslinking, the biomimetic microchannels are expected to be used in the bionic reconstruction of solid functional organs.
【作者单位】： 西安交通大学机械制造系统工程国家重点实验室;
【基金】：国家自然科学基金资助项目(51675416) 陕西省统筹项目(2015KTTSGY-04)
【分类号】：R318.08
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