硫化氢通过缺氧诱导因子1α促进张应力下骨髓间充质细胞的成骨

发布时间：2018-09-08 21:15

【摘要】：背景:有研究表明,硫化氢信号能够促进牵张成骨,而具体机制尚不清楚。目的:观察硫化氢气体信号在促进张应力下大鼠骨髓间充质细胞成骨分化过程中与缺氧诱导因子1α的关系。方法:应用四点弯曲细胞加力装置系统,对体外分离培养的大鼠骨髓间充质细胞施加2 000μstrain的单一周期张应力,同时通过使用工具药物改变加力单元中硫化氢及缺氧诱导因子1α的含量水平,加力后检测成骨标志物的变化。结果与结论:(1)细胞成骨:外源性硫化氢信号能够促进张应力下大鼠骨髓间充质细胞成骨,而抑制加力单元缺氧诱导因子1α的表达则会减弱硫化氢信号的促进成骨作用;(2)结果说明:硫化氢信号系统可能通过缺氧诱导因子1α促进张应力下大鼠大鼠骨髓间充质细胞成骨作用。
[Abstract]:Background: hydrogen sulfide signals have been shown to promote distraction osteogenesis, but the mechanism is unclear. Aim: to investigate the relationship between hydrogen sulfide gas signal and hypoxia inducible factor-1 伪 in promoting osteogenic differentiation of bone marrow mesenchymal cells under tensile stress in rats. Methods: a four-point bending cell system was used to apply a single cycle tensile stress of 2 000 渭 strain to rat bone marrow mesenchymal cells isolated and cultured in vitro. At the same time, the levels of hydrogen sulfide and anoxia-inducible factor-1 伪 were changed by the use of tool drugs, and the changes of osteoblastic markers were detected. Results and conclusion: (1) Osteogenesis: exogenous hydrogen sulfide signal could promote osteogenesis of bone marrow mesenchymal cells under tensile stress in rats. However, inhibiting the expression of hypoxia inducible factor-1 伪 in the afterloading unit could attenuate the osteogenic effect of hydrogen sulfide signal. (2) the results suggest that the hydrogen sulfide signaling system may promote bone marrow mesenchyma under tension stress through hypoxia inducible factor 1 伪. Osteogenesis of mesenchymal cells.
【作者单位】：郴州市第一人民医院口腔科南华大学转化医学研究所;
【基金】：国家自然科学基金(81301651) 郴州市第一人民医院重点项目(N2015-006)~~
【分类号】：R318

【参考文献】