钛金属表面飞秒激光掺银改性的生物学研究
发布时间：2018-09-09 09:12
【摘要】：钛及钛合金以其优良的物理化学性能(如较强的耐腐蚀性,较高的韧性和熔点及质量较轻等)在种植牙领域得到广泛应用,但可能由于种植体周围发生感染以及缺乏良好的骨-种植体整合而导致种植体植入失败。研究表明,通过对种植体表面改性可提高种植牙的成功率。近年来,研究的重点主要集中在以下一些表面改性方法：通过改善表面可湿性、设计表面粗糙度及形貌、制备与骨成分相近的涂层等方法提高种植体与周围骨组织整合；通过种植体表面修饰抗菌剂或制备功能性涂层作为抗菌元素的载体等方式降低种植体周围感染的发生率。但是,既能促进骨细胞增殖同时降低种植体周围感染发生的简单、有效的表面改性方法目前还未见报道。 在本研究中,我们利用飞秒激光蒸镀掺银改性技术对种植体表面进行改性,期望促进种植体周围骨细胞增殖的同时降低种植体周围感染的发生率。飞秒激光以其高精确度、表面形貌可控性、无污染等特性在材料(金属、半导体以及陶瓷材料等)表面改性领域得到广泛应用,能够有效改变材料的光学、电学、力学及磁学性能。在本研究中,将飞秒激光应用于对钛及钛合金的表面改性中,研究飞秒激光诱导材料表面形成不同的周期性结构及其对细胞增殖和抗菌性的影响。通过改变飞秒激光能量参数,钛表面形成不同尺度的周期性结构：当激光的能量密度为0.07J/cm2时,钛表面形成周期性纳米级条纹结构；当激光的能量密度为1.4J/cm2时,钛表面形成微米级凸起与纳米级条纹叠加的复合结构,将其与细胞共培养,我们发现微纳米复合结构表面有利于成骨细胞的黏附和增殖。我们又将飞秒激光技术与蒸镀掺银技术结合,在制备微纳米复合结构的同时实现对种植体表面的可控性银掺杂,提高了种植体的生物相容性及抗菌性。细胞毒性实验结果显示,在银的掺杂量较小时,种植体没有细胞毒性,并有一定的促进细胞增殖的作用。抗菌性的研究结果表明,随着银含量的增加,种植体的抗菌能力逐渐增加。 综上所述,我们利用飞秒激光蒸镀掺银改性技术对钛种植体表面进行形貌改变及银的可控性掺杂,并进行体外实验,结果证实飞秒激光加工形成的微纳米复合结构提高了钛的生物相容性；掺杂适当的银可以促进细胞的黏附和增殖,提高了钛的生物相容性,钛的抗菌性随着银含量的增加逐渐增强。以上结果表明飞秒激光蒸镀掺银技术可达到促进成骨细胞的增殖,提高种植材料的生物相容性,同时降低细菌感染的双重作用,为钛种植体的体内实验及临床应用奠定了基础。
[Abstract]:Titanium and titanium alloys are widely used in the field of dental implants for their excellent physical and chemical properties, such as strong corrosion resistance, higher toughness, lower melting point and lower mass. However, implants may fail due to infection around implants and lack of good osseous-implant integration. The results showed that the success rate of implant teeth could be improved by surface modification of implants. In recent years, the research focuses on the following methods of surface modification: improving surface wettability, designing surface roughness and morphology, preparing coatings similar to bone composition, improving the integration of implants and surrounding bone tissues; The incidence of peri-implant infection was reduced by surface modification of antibacterial agent or preparation of functional coating as carrier of antimicrobial elements. However, a simple and effective surface modification method has not been reported, which can not only promote the proliferation of bone cells but also reduce the incidence of peri-implant infection. In this study, we modified the implant surface by femtosecond laser evaporation with silver doping, in order to promote the proliferation of bone cells around the implant and reduce the incidence of infection around the implant. Femtosecond laser has been widely used in the field of surface modification of materials (metal, semiconductor, ceramic, etc.) because of its high accuracy, controllable surface morphology and no pollution, which can effectively change the optical and electrical properties of materials. Mechanical and magnetic properties. In this study, femtosecond laser was applied to the surface modification of titanium and titanium alloys. The effects of femtosecond laser on the formation of different periodic structures on the surface of titanium and titanium alloys and their effects on cell proliferation and antibacterial activity were studied. By changing the energy parameters of femtosecond laser, different scale periodic structures are formed on the titanium surface: when the laser energy density is 0.07J/cm2, the titanium surface forms periodic nanoscale stripe structure, and when the laser energy density is 1.4J/cm2, The composite structure of micrometer protuberance and nanometer stripe was formed on the surface of titanium and co-cultured with cells. We found that the surface of micro-nano composite structure is conducive to the adhesion and proliferation of osteoblasts. We also combine femtosecond laser technology with vaporizing silver-doped technology to fabricate micro-nano composite structure and achieve controllable silver doping on implant surface, which improves the biocompatibility and antibacterial property of implant. The results of cytotoxicity test showed that the implants had no cytotoxicity and promoted the proliferation of cells in a small amount of silver doping. The results showed that the antibacterial ability of the implants increased with the increase of silver content. To sum up, we use femtosecond laser vaporizing silver-doped technology to modify the surface morphology of titanium implants and the controllable doping of silver, and conduct experiments in vitro. The results show that the microcomposite structure formed by femtosecond laser processing can improve the biocompatibility of titanium, and the addition of appropriate silver can promote cell adhesion and proliferation, and improve the biocompatibility of titanium. The antibacterial activity of titanium increases with the increase of silver content. The results show that femtosecond laser evaporation and silver doping can promote the proliferation of osteoblasts, improve the biocompatibility of implant materials, and reduce the double effect of bacterial infection, which lays a foundation for the in vivo experiment and clinical application of titanium implants.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R783.1
本文编号：2231951
[Abstract]:Titanium and titanium alloys are widely used in the field of dental implants for their excellent physical and chemical properties, such as strong corrosion resistance, higher toughness, lower melting point and lower mass. However, implants may fail due to infection around implants and lack of good osseous-implant integration. The results showed that the success rate of implant teeth could be improved by surface modification of implants. In recent years, the research focuses on the following methods of surface modification: improving surface wettability, designing surface roughness and morphology, preparing coatings similar to bone composition, improving the integration of implants and surrounding bone tissues; The incidence of peri-implant infection was reduced by surface modification of antibacterial agent or preparation of functional coating as carrier of antimicrobial elements. However, a simple and effective surface modification method has not been reported, which can not only promote the proliferation of bone cells but also reduce the incidence of peri-implant infection. In this study, we modified the implant surface by femtosecond laser evaporation with silver doping, in order to promote the proliferation of bone cells around the implant and reduce the incidence of infection around the implant. Femtosecond laser has been widely used in the field of surface modification of materials (metal, semiconductor, ceramic, etc.) because of its high accuracy, controllable surface morphology and no pollution, which can effectively change the optical and electrical properties of materials. Mechanical and magnetic properties. In this study, femtosecond laser was applied to the surface modification of titanium and titanium alloys. The effects of femtosecond laser on the formation of different periodic structures on the surface of titanium and titanium alloys and their effects on cell proliferation and antibacterial activity were studied. By changing the energy parameters of femtosecond laser, different scale periodic structures are formed on the titanium surface: when the laser energy density is 0.07J/cm2, the titanium surface forms periodic nanoscale stripe structure, and when the laser energy density is 1.4J/cm2, The composite structure of micrometer protuberance and nanometer stripe was formed on the surface of titanium and co-cultured with cells. We found that the surface of micro-nano composite structure is conducive to the adhesion and proliferation of osteoblasts. We also combine femtosecond laser technology with vaporizing silver-doped technology to fabricate micro-nano composite structure and achieve controllable silver doping on implant surface, which improves the biocompatibility and antibacterial property of implant. The results of cytotoxicity test showed that the implants had no cytotoxicity and promoted the proliferation of cells in a small amount of silver doping. The results showed that the antibacterial ability of the implants increased with the increase of silver content. To sum up, we use femtosecond laser vaporizing silver-doped technology to modify the surface morphology of titanium implants and the controllable doping of silver, and conduct experiments in vitro. The results show that the microcomposite structure formed by femtosecond laser processing can improve the biocompatibility of titanium, and the addition of appropriate silver can promote cell adhesion and proliferation, and improve the biocompatibility of titanium. The antibacterial activity of titanium increases with the increase of silver content. The results show that femtosecond laser evaporation and silver doping can promote the proliferation of osteoblasts, improve the biocompatibility of implant materials, and reduce the double effect of bacterial infection, which lays a foundation for the in vivo experiment and clinical application of titanium implants.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R783.1
【参考文献】
相关期刊论文 前4条
1 ;Preparation of porous microstructures on NiTi alloy surface with femtosecond laser pulses[J];Chinese Science Bulletin;2008年05期
2 贺刚;陈治清;盛祖立;;纯钛种植体表面纳米含氟磷灰石涂层的构建和表征[J];中国口腔种植学杂志;2007年02期
3 贾爽;范震;苏剑生;;种植体表面粗糙度对骨结合影响的研究现状[J];中国口腔种植学杂志;2007年02期
4 王珏;焦艳军;;种植体周围炎的微生物学研究[J];中国口腔种植学杂志;2008年01期
，本文编号：2231951
本文链接：https://www.wllwen.com/yixuelunwen/swyx/2231951.html
